ABCB1 p.Leu70Arg
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PMID: 18596043
[PubMed]
Loo TW et al: "Arginines in the first transmembrane segment promote maturation of a P-glycoprotein processing mutant by hydrogen bond interactions with tyrosines in transmembrane segment 11."
No.
Sentence
Comment
117
Introduction of arginines at other positions at the extracellular end of TM1 had little effect (10-20% mature P-gp in mutants L67R, M69R, and L70R) or reduced the level of mature product to less than 10% of total P-gp (mutants P66R and F72R).
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ABCB1 p.Leu70Arg 18596043:117:142
status: NEW150 Mutants V52R, L67R, and L70R resembled the parent (G251V) P-gp, as expression in any of the drug substrates promoted efficient maturation (mature P-gp became the major product) of the protein.
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ABCB1 p.Leu70Arg 18596043:150:24
status: NEW165 TABLE 1 Effects of drug substrates on the maturation of TM1 arginine mutants containing the G251V mutation Mutation (G251V ؉) No drug Cyclosporin A Verapamil Vinblastine Rhodamine None - 111a,c 111 111 11 V52R -a,b 111 111 111 11 V53R 2 - - - - G64R 2 - - - - T55R 2 - - - - L56R 2 - - - - A57R 2 - - - - A58R 2 - - - - I59R 2 - - - - I60R 2 - - - - H61R - 111 1 1 1 G62R 2 - - - - A63R 2 - - - - G64R 1 111 1 1 11 L65R 11 111 11 11 11 P66R 2 1 - - - L67R - 111 111 111 11 M68R 111 111 111 111 111 M69R - 111 - 11 111 L70R - 111 111 111 11 V71R 1 111 111 111 11 F72R 2 1 1 1 1 a Change in the amount of mature (170 kDa) protein in the presence of drug substrate relative to that in the absence of drug substrate.
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ABCB1 p.Leu70Arg 18596043:165:524
status: NEW