ABCB1 p.Met69Cys

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PMID: 16042402 [PubMed] Loo TW et al: "ATP hydrolysis promotes interactions between the extracellular ends of transmembrane segments 1 and 11 of human multidrug resistance P-glycoprotein."
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97 Figure 2A shows that the slower migrating product in mutants M68C(TM1)/Y950C(TM11), M68C- (TM1)/Y953C(TM11), M68C(TM1)/A954C(TM11), M69C- (TM1)/A954C(TM11), and M69C(TM1)/F957C(TM11) was greatly reduced upon exposure to 10 mM dithiothreitol.
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ABCB1 p.Met69Cys 16042402:97:132
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105 Mutants M68C(TM1)/ Y950C(TM11), M68C(TM1)/Y953C(TM11), M68C(TM1)/ A954C(TM11), M69C(TM1)/A954C(TM11), and M69C- (TM1)/F957C(TM11) all showed a time-dependent increase FIGURE 1: Model of packing of the TM segments of P-gp and location of the introduced cysteines.
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ABCB1 p.Met69Cys 16042402:105:106
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134 Membranes were prepared from HEK 293 cells (A), expressing mutants M68C(TM1)/Y950C(TM11), M68C(TM1)/Y953C(TM11), M68C- (TM1)/A954C(TM11), M69C(TM1)/A954C(TM11), M69C(TM1)/ F957C(TM11), or the negative mutant F72C(TM1)/F957C(TM11); (B) the single cysteine mutants M68C(TM1), M69C(TM1), Y950C(TM11), Y953C(TM11), A954C(TM11), or F957C(TM11); or (C) coexpressing the single cysteine mutants M68C(TM1) plus Y950C(TM11), M68C(TM1) plus Y953C(TM11), M68C(TM1) plus A954C(TM11), M69C(TM1) plus A954C(TM11), or M69C- (TM1) plus F957C(TM11).
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ABCB1 p.Met69Cys 16042402:134:503
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163 Accordingly, the histidine-tagged P-gp mutants M68C(TM1)/ Y950C(TM11), M68C(TM1)/Y953C(TM11), M68C(TM1)/ A954C(TM11), M69C(TM1)/A954C(TM11), and M69C- (TM1)/F957C(TM11) and the Cys-less P-gp were expressed in HEK 293 cells and isolated by nickel-chelate chromatography.
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ABCB1 p.Met69Cys 16042402:163:145
status: NEW
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