ABCB1 p.Phe1268Ala

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PMID: 15542593 [PubMed] Loo TW et al: "The dileucine motif at the COOH terminus of human multidrug resistance P-glycoprotein is important for folding but not activity."
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6 By contrast, mutants L1261A, F1268A, and wild-type P-gps expressed the 170-kDa mature proteins at the cell surface.
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ABCB1 p.Phe1268Ala 15542593:6:29
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90 By contrast, the major product in wild-type P-gp and in mutants L1261A, K1264A, and F1268A was the 170-kDa protein.
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ABCB1 p.Phe1268Ala 15542593:90:84
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106 Accordingly, wild-type P-gp and mutants L1260A, L1261A, K1264A, Y1267A, and F1268A were expressed in HEK 293 cells in the presence or absence of the chemical chaperone/drug substrate cyclosporin A. Whole cell SDS extracts were then subjected to immunoblot analysis. Fig. 2A shows that the cyclosporin A was able to rescue mutants L1260A and Y1267A, because a 170-kDa protein was then detected (Fig. 2A).
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ABCB1 p.Phe1268Ala 15542593:106:76
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