ABCMdb

ABCB1 p.Ser1177Cys

None has been submitted yet.

Publications

PMID: 12226074 [PubMed] Loo TW et al: "The "LSGGQ" motif in each nucleotide-binding domain of human P-glycoprotein is adjacent to the opposing walker A sequence."

No. Sentence Comment

80 Mutants L1176C/S429C and L1176C/G432C were cross-linked only at 21 and 37 °C, while cross-linking of mutants L1176C/G430C, S1177C/S429C, S1177C/G430C, S1177C/ C431, S1177C/G432C, and G1179C/S429C was only observed at 37 °C. Login to comment
92 S429C, S1177C/S429C, L1176C/C431, and S1177C/C431, was inhibited by preincubation with ATP plus vanadate (Table II). Login to comment
94 No inhibition of cross-linking by vanadate trapping was observed in mutants such as L1176C/G430C, L1176C/G432C, S1177C/G430C, or S1177C/G432C. Login to comment
124 Lines indicate residues cross-linked at 4 °C. TABLE II Cross-linking between residues in the NBD2 signature sequence and in the NBD1 Walker A site L1176C (53%)a S1177C (35%) G1178C (78%) G1179C (14%) Q1180C (23%) 4 °C 21 °C 37 °C 4 °C 21 °C 37 °C 4 °C 21 °C 37 °C 4 °C 21 °C 37 °C 4 °C 21 °C 37 °C S429C (38%) -b *c ** - - * - - - - - ϩ - - - G430C (12%) - - ϩϩd - - ϩ - - - - - - - - - C431 (100%) * ** **d,e - - * - - - - - - - - - G432C (0%) - ϩc ϩϩ - - ϩ - - - - - - - - - K433C (12%) - - - - - - - - - - - - - - - a Activity of the single cysteine mutant relative to Cys-less P-gp. b No cross-linked product detected in SDS-PAGE. c Relatively weak cross-linking (Ͻ50% of P-gp cross-linked). Login to comment