ABCB1 p.Gly533Cys
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PMID: 12226074
[PubMed]
Loo TW et al: "The "LSGGQ" motif in each nucleotide-binding domain of human P-glycoprotein is adjacent to the opposing walker A sequence."
No.
Sentence
Comment
59
The cysteines in other mutants (e.g. L531C/S1072C, S532/S1072C, and G533C/G1073C) must be further apart, since cross-linked product was only observed at either 21 or 37 °C.
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ABCB1 p.Gly533Cys 12226074:59:68
status: NEW70 Similar results were observed in mutants L531C/ S1071C, S532C/C1074, G533C/C1074C, and L531C/K1076C (Table I).
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ABCB1 p.Gly533Cys 12226074:70:69
status: NEW71 Mutants L531C/C1074, L531C/S1071C, S532C/ C1074, G533C/C1074, and L531C/K1076C had verapamil-stimulated ATPase activities of 22, 22, 30, 90, and 11% respectively, relative to Cys-less P-gp.
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ABCB1 p.Gly533Cys 12226074:71:49
status: NEW89 CP, copper phenanthroline TABLE I Cross-linking between residues in the NBD1 signature sequence and in the NBD2 Walker A site L531C (21%)a S532C (38%) G533C (91%) G534C (4%) Q535C (0%) 4 °C 21 °C 37 °C 4 °C 21 °C 37 °C 4 °C 21 °C 37 °C 4 °C 21 °C 37 °C 4 °C 21 °C 37 °C G1070C (0%) -b - - - - - - - - - - - - - - S1071C (85%) - - *c - - - - - - - - ϩ - - ϩ S1072C (23%) - ϩc ϩϩd - ϩϩ ϩϩ - - ϩ - - ϩ - - - G1073C (4%) ϩ ϩϩ ϩϩ ϩ ϩϩ ϩϩ - ϩ ϩϩ - - - - - - C1074 (103%) ** ** **d,e - * * - - * - - - - - - G1075C (0%) - - ϩϩ - - ϩ - - - - - - - - - K1076C (12%) - - * - - - - - - - - - - - - S1077C (0%) - - - - - - - - - - - - - - - a Activity of the single cysteine mutant relative to Cys-less P-gp. b No cross-linked product detected in SDS-PAGE. c Relatively weak cross-linking (Ͻ50% of P-gp cross-linked).
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ABCB1 p.Gly533Cys 12226074:89:151
status: NEW