ABCB1 p.Ser1072Cys
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PMID: 12226074
[PubMed]
Loo TW et al: "The "LSGGQ" motif in each nucleotide-binding domain of human P-glycoprotein is adjacent to the opposing walker A sequence."
No.
Sentence
Comment
50
The cross-linking results of three mutants, L531C/G1070C, L531C/S1072C, and L531C/C1074, are shown in Fig. 1.
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ABCB1 p.Ser1072Cys 12226074:50:64
status: NEW52 In mutant L531C/S1072C, no cross-linked product was detected when treated with oxidant at 4 °C.
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ABCB1 p.Ser1072Cys 12226074:52:16
status: NEW59 The cysteines in other mutants (e.g. L531C/S1072C, S532/S1072C, and G533C/G1073C) must be further apart, since cross-linked product was only observed at either 21 or 37 °C.
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ABCB1 p.Ser1072Cys 12226074:59:43
status: NEWX
ABCB1 p.Ser1072Cys 12226074:59:56
status: NEW84 Membranes were prepared from HEK 293 cells expressing mutants L531C/G1070C, L531C/ S1072C, or L531C/C1074.
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ABCB1 p.Ser1072Cys 12226074:84:83
status: NEW89 CP, copper phenanthroline TABLE I Cross-linking between residues in the NBD1 signature sequence and in the NBD2 Walker A site L531C (21%)a S532C (38%) G533C (91%) G534C (4%) Q535C (0%) 4 °C 21 °C 37 °C 4 °C 21 °C 37 °C 4 °C 21 °C 37 °C 4 °C 21 °C 37 °C 4 °C 21 °C 37 °C G1070C (0%) -b - - - - - - - - - - - - - - S1071C (85%) - - *c - - - - - - - - ϩ - - ϩ S1072C (23%) - ϩc ϩϩd - ϩϩ ϩϩ - - ϩ - - ϩ - - - G1073C (4%) ϩ ϩϩ ϩϩ ϩ ϩϩ ϩϩ - ϩ ϩϩ - - - - - - C1074 (103%) ** ** **d,e - * * - - * - - - - - - G1075C (0%) - - ϩϩ - - ϩ - - - - - - - - - K1076C (12%) - - * - - - - - - - - - - - - S1077C (0%) - - - - - - - - - - - - - - - a Activity of the single cysteine mutant relative to Cys-less P-gp. b No cross-linked product detected in SDS-PAGE. c Relatively weak cross-linking (Ͻ50% of P-gp cross-linked).
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ABCB1 p.Ser1072Cys 12226074:89:444
status: NEW