ABCB1 p.Gln195Cys
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PMID: 10681495
[PubMed]
Loo TW et al: "The packing of the transmembrane segments of human multidrug resistance P-glycoprotein is revealed by disulfide cross-linking analysis."
No.
Sentence
Comment
77
In these cross-linking experiments, the amount of oxidant was lowered by 10-fold (0.2 mM), and the minimum temperature required to induce cross-TABLE I Cross-linking analysis of P-gp Cross-linking of S993C (TM12) with residues in the following TM: TM1 TM2 TM3 TM4 TM5 M51C -a Y130C - G185C - G226C - I293C - V52C - I131C - I186C - L227C ϩb T294C - V53C - Q132C - G187C - S228C - A295C ϩ G54C - V133C - D188C - A229C - N296C - T55C - S134C - K189C - A230C - I297C - L56C - F135C - I190C - V231C ϩ S298C - A57C - W136C - G191C - W232C ϩ I299C ϩ A58C - C137C - M192C - A233C ϩ G300C - I59C - L138C - F193C - K234C - A301C - I60C - A139C - F194C - I235C ϩ A302C - H61C - A140C - Q195C - L236C ϩ F303C - G141C - S196C - S237C - L304C - Cross-linking of P350C (TM6) with residues in the following TM: TM7 TM8 TM9 TM10 TM11 F711C - F770C - A828C - I867C - A935C - V712C - F771C - I829C - I868C - H936C - V713C - L772C - G830C - A869C - I937C - G714C - Q773C - S831C - I870C - F938C - V715C - G774C - R832C - A871C - G939C ϩ F716C - F775C - L833C - G872C - I940C - C717C - T776C - A834C - V873C - T941C - A718C - F777C - V835C - V874C ϩ F942C - I719C - G778C - I836C - E875C ϩ S943C - I720C - K779C - T837C - M876C ϩ F944C - N721C - A780C - Q838C - K877C - T945C - G722C - G781C - N839C - M878C - Q946C - G723C - E782C - I840C - L879C - A947C - I783C - a -, no cross-linked product detected in SDS-PAGE. b ϩ, cross-linked product detected in SDS-PAGE.
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ABCB1 p.Gln195Cys 10681495:77:717
status: NEW
PMID: 12223492
[PubMed]
Loo TW et al: "Location of the rhodamine-binding site in the human multidrug resistance P-glycoprotein."
No.
Sentence
Comment
133
In TM3, the activities of two mutants (K189C and Q195C) were inhibited 76 and 78%, respectively, by MTS-rhodamine.
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ABCB1 p.Gln195Cys 12223492:133:49
status: NEW
PMID: 17848563
[PubMed]
Loo TW et al: "Suppressor mutations in the transmembrane segments of P-glycoprotein promote maturation of processing mutants and disrupt a subset of drug-binding sites."
No.
Sentence
Comment
154
The mutants Q195C and T199C, however, exhibited different properties than Cys-less P-gp after treatment with MTS-rhodamine.
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ABCB1 p.Gln195Cys 17848563:154:12
status: NEW155 Whereas mutant Q195C showed a 6.4-fold stimulation of ATPase activity with rhodamine B before treatment with MTS-rhodamine, its activity could only be stimulated 1.3-fold after treatment with MTS-rhodamine.
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ABCB1 p.Gln195Cys 17848563:155:15
status: NEW168 Fold-stimulation 1 2 3 4 5 6 Cys-less I190C G191C M192C F193C F194C Q195C S196C M197C A198C T199C F200C F201C T202C G203C F204C I205C V206C G207C F208C T209C A 7 Fold-stimulation 1 2 3 4 5 6 Cys-less B _ + MTS-rhod Rhod B+ _ + Q195C + + _ + T199C + + _ + _ _+ + + 7 FIGURE 2.
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ABCB1 p.Gln195Cys 17848563:168:68
status: NEWX
ABCB1 p.Gln195Cys 17848563:168:227
status: NEW173 B, Cys-less, Q195C and T199C P-gp mutants were treated with (ϩ) or without (-) 2 mM MTS-rhodamine and histidine-tagged P-gp isolated by nickel-chelate chromatography. Equivalent amounts of P-gp were mixed with lipid, and ATPase activity was determined in the presence (ϩ) or absence (-) of 2 mM rhodamine B (Rhod B).
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ABCB1 p.Gln195Cys 17848563:173:13
status: NEW