ABCB1 p.Tyr710Ala
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PMID: 10506575
[PubMed]
Loo TW et al: "The human multidrug resistance P-glycoprotein is inactive when its maturation is inhibited: potential for a role in cancer chemotherapy."
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64
Accordingly, the histidine-tagged 150 kDa protein of mutants G268V, G269V, and Y710A were isolated (Fig. 1, lanes 1, 3, and 5).
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ABCB1 p.Tyr710Ala 10506575:64:79
status: NEW66 To convert the processing mutants G268V, G269V, and Y710A to the mature enzyme, the mutants were synthesized in the presence of 10 M cyclosporin A to induce maturation (19).
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ABCB1 p.Tyr710Ala 10506575:66:52
status: NEW72 The mature enzyme of mutant Y710A had activities that were similar to wild-type P-gp.
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ABCB1 p.Tyr710Ala 10506575:72:28
status: NEW76 ATPase activity of P-gp that is prevented from undergoing maturation It was possible that the 150 kDa core-glycosylated P-gp of the processing mutants G268V, G269V, and Y710A were inactive because the point mutations inherently caused misfolding of the protein and subsequent retention in the endoplasmic reticulum.
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ABCB1 p.Tyr710Ala 10506575:76:169
status: NEW84 Equivalent amounts of histidine-tagged mature and core-glycosylated P-gp of wild-type and mutants G268V, G269V, and Y710A, isolated by nickel-chelate chromatography, were reconstituted with lipid and assayed for verapamil- (1 mM) and vinblastine- (0.05 mM) stimulated ATPase activities.
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ABCB1 p.Tyr710Ala 10506575:84:116
status: NEW88 HEK293 cells expressing histidine-tagged P-gp mutants G268V, G269V or Y710A were incubated with (ϩ) or without (-) 10 M cyclosporin A (cyclo) for 24 h. The cells were harvested and solubilized with n-dodecyl-beta-D-maltoside and the cell extracts were subjected to nickel-chelate chromatography with 20 mM imidazole in the wash buffers.
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ABCB1 p.Tyr710Ala 10506575:88:70
status: NEW