ABCC7 p.Thr339Ser
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PMID: 9729613
[PubMed]
Linsdell P et al: "Non-pore lining amino acid side chains influence anion selectivity of the human CFTR Cl- channel expressed in mammalian cell lines."
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61
However, following immunoprecipitation of CFTR protein from five confluent plates, a very small amount of T339S mutant CFTR protein was detectable by Western blotting (Fig. 1E), indicating that the BHK cells had been successfully transfected with the mutated plasmid, but that most mutations at T339 caused more severe misprocessing andÏor degradation of the CFTR protein.
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ABCC7 p.Thr339Ser 9729613:61:106
status: NEW81 E, Western blot of immunoprecipitated T339S protein, carried out as described in Methods.
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ABCC7 p.Thr339Ser 9729613:81:38
status: NEW174 Indeed, we found no PKAand ATP-dependent currents in very large inside-out patches excised from BHK cells expressing T339A (n = 6), T339S (n = 4), T339Y (n = 6) or T339F (n = 5) using symmetrical 150 mÒ NaCl-containing solutions (not shown).
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ABCC7 p.Thr339Ser 9729613:174:132
status: NEW