ABCC7 p.Thr338Phe
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PMID: 9729613
[PubMed]
Linsdell P et al: "Non-pore lining amino acid side chains influence anion selectivity of the human CFTR Cl- channel expressed in mammalian cell lines."
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59
Stable expression of all six T338 mutants constructed in BHK cells led to the production of both core-glycosylated (band B) and fully glycosylated (band C) CFTR protein, although some mutants (especially T338F) P. Linsdell, S.-X. Zheng and J. W. Hanrahan J. Physiol. 512.12 ) at UNIV OF NORTH CAROLINA on August 8, consistently made less band C protein than wild-type (Fig. 1C).
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ABCC7 p.Thr338Phe 9729613:59:204
status: NEW63 In contrast, no currents were activated under these conditions in large inside-out patches excised from BHK cells expressing T338F (n = 8; not shown).
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ABCC7 p.Thr338Phe 9729613:63:125
status: NEW118 Thus, the lack of macroscopic Cl¦ currents in membrane patches excised from BHK cells expressing T338F (see above) may reflect a non-conducting phenotype for this mutant.
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ABCC7 p.Thr338Phe 9729613:118:102
status: NEW119 Alternatively, misprocessing or degradation of T338F protein (Fig. 1C) may result in expression levels below our threshold of detection using patch clamp recording.
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ABCC7 p.Thr338Phe 9729613:119:47
status: NEW