ABCC7 p.Tyr512Glu
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PMID: 21807898
[PubMed]
Mendes AI et al: "Antagonistic Regulation of Cystic Fibrosis Transmembrane Conductance Regulator Cell Surface Expression by Protein Kinases WNK4 and Spleen Tyrosine Kinase."
No.
Sentence
Comment
10
Mutation of Tyr512 to either glutamic acid or37 phenylalanine is sufficient to alter CFTR surface levels.
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ABCC7 p.Tyr512Glu 21807898:10:12
status: NEW138 CFTR-Y512E expressing cells showed a significant506 decrease in CFTR-mediated ion transport while cells expressing CFTR Y512F507 released almost twice the amount of iodide as wt CFTR-expressing cells.
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ABCC7 p.Tyr512Glu 21807898:138:5
status: NEW142 Second, cells were co-transfected with the phospho-mimetic CFTR-516 Y512E mutant and Myc-WNK4-wt.
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ABCC7 p.Tyr512Glu 21807898:142:68
status: NEW143 Under these conditions, the expression of517 WNK4 was unable to rescue surface levels of the CFTR-Y512E mutant protein518 (FIG. 8B, two right lanes) to those of wt CFTR (second lane from left).519 520 521 DISCUSSION522 523 The data presented in this work provide two novel insights into the regulation of524 cell surface expression of CFTR.
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ABCC7 p.Tyr512Glu 21807898:143:98
status: NEW