ABCC7 p.Lys946Arg

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PMID: 21483833 [PubMed] Drevillon L et al: "COMMD1-mediated ubiquitination regulates CFTR trafficking."
No. Sentence Comment
114 Three lysines were mutated to arginine in the ICL3 domain near Ser-945 (K946R and K951R) and Asp-979 (K978R).
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ABCC7 p.Lys946Arg 21483833:114:72
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116 When COMMD1 was overexpressed, ubiquitination of K946R- and K951R-CFTR was increased (15263% and 126615%, respectively) whereas ubiquitination of wt-CFTR was decreased (70615%) (Figure 5A and 5B).
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ABCC7 p.Lys946Arg 21483833:116:49
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119 However, ubiquitination of K946R-CFTR and K951R-CFTR was significantly increased compared to that of wt-CFTR (Figure 5B).
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ABCC7 p.Lys946Arg 21483833:119:27
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172 (A) HeLa cells were transfected with CFTR constructs (wt, K946R, K951R or K978R-CFTR) and Myc-COMMD1 or empty vector as control (mock).
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ABCC7 p.Lys946Arg 21483833:172:58
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180 (C) Representative gels for the same co-immunoprecipitation experiment between COMMD1 and wt, K946R, K951R or K978R-CFTR in heterologous system. HeLa cells were co-transfected with Myc-COMMD1 or empty vector (mock) and wt, K946R, K951R or K978R-CFTR.
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ABCC7 p.Lys946Arg 21483833:180:94
status: NEW
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ABCC7 p.Lys946Arg 21483833:180:223
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185 COMMD1 overexpression clearly inhibited wt-CFTR ubiquitination, whereas it strongly induced ubiquitination of K946R- and K951R-CFTR.
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ABCC7 p.Lys946Arg 21483833:185:110
status: NEW
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