ABCC7 p.Asp836Ala
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PMID: 20952391
[PubMed]
Wang G et al: "State-dependent regulation of cystic fibrosis transmembrane conductance regulator (CFTR) gating by a high affinity Fe3+ bridge between the regulatory domain and cytoplasmic loop 3."
No.
Sentence
Comment
132
Fig. 4, B and E, indicate that only D836A dramatically prevented inhibition by Fe3ϩ , whereas E822A, E826A, D828A, E831A, and D835A did not.
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ABCC7 p.Asp836Ala 20952391:132:36
status: NEW133 Similarly, activity of the D836A mutant was also potentiated by curcumin dramatically (Fig. 4B).
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ABCC7 p.Asp836Ala 20952391:133:27
status: NEW145 In support of this proposal, H950A/H954A and D836A/C832A/ H774A completely prevented Fe3ϩ inhibition, which was reversed by EDTA (Fig. 4E).
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ABCC7 p.Asp836Ala 20952391:145:45
status: NEW203 B-D, macroscopic currents across inside-out membrane patches excised from transfected HEK-293T cells expressing the D836A mutant (B), the mouse CFTR (mCFTR) (C), and the H950A mutant (D).
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ABCC7 p.Asp836Ala 20952391:203:116
status: NEW
PMID: 23060444
[PubMed]
Wang G et al: "Regulation of Activation and Processing of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by a Complex Electrostatic Interaction between the Regulatory Domain and Cytoplasmic Loop 3."
No.
Sentence
Comment
11
First, not only D835A, D836A and E838A but also K946A reduced the PKA dependent CFTR activation.
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ABCC7 p.Asp836Ala 23060444:11:23
status: NEW138 First, the PKA sensitivity of channel activation was significantly enhanced for K946A, D835A, D836A and E838A mutants (Fig.2).
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ABCC7 p.Asp836Ala 23060444:138:94
status: NEW9 First, not only D835A, D836A, and E838A but also K946A reduced the PKA-dependent CFTR activation.
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ABCC7 p.Asp836Ala 23060444:9:23
status: NEW62 In contrast, mutation of Lys-946 or Asp-836 to alanine clearly accelerated the channel activation and reduced the PKA dependence (Fig. 2, B and C).
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ABCC7 p.Asp836Ala 23060444:62:36
status: NEW98 The PKA-dependent activity of CFTR mutants at the R-CL3 interface. A-C, macroscopic currents across inside-out membrane patches excised from transfected HEK-293T cells expressing WT CFTR (A) and mutants K946A (B) and D836A (C) by using a ramp protocol (afe;80 mV).
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ABCC7 p.Asp836Ala 23060444:98:217
status: NEW167 First, the PKA sensitivity of channel activation was significantly enhanced for K946A, D835A, D836A, and E838A mutants (Fig. 2).
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ABCC7 p.Asp836Ala 23060444:167:94
status: NEW