ABCC7 p.Lys978Gln
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PMID: 20926782
[PubMed]
Li MS et al: "Regulation of CFTR chloride channel macroscopic conductance by extracellular bicarbonate."
No.
Sentence
Comment
118
To investigate whether these positively charged residues influence the strength of channel block by cytosolic anions in intact cells, we neutralized these charges in an E1371Q background.
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ABCC7 p.Lys978Gln 20926782:118:82
status: NEW119 Figure 6A shows macroscopic currents carried by K95Q/ E1371Q-, R303Q/E1371Q-, and K978Q/E1371Q-CFTR in Fig. 4.
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ABCC7 p.Lys978Gln 20926782:119:82
status: NEW
PMID: 17582383
[PubMed]
Melin P et al: "CFTR inhibition by glibenclamide requires a positive charge in cytoplasmic loop three."
No.
Sentence
Comment
2
Charge-neutralizing mutations K978A, K978Q, K978S abolished the inhibition of forskolin-activated CFTR chloride current by glibenclamide but not by CFTRinh-172.
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ABCC7 p.Lys978Gln 17582383:2:37
status: NEW77 We investigated the role of the charged residue K978 in the sequence ILNRFSKD of CL3 (Fig. 1B) described as a region physically close to the pore [29] and examined the interaction of glibenclamide with mutated EGFP-CFTR channels: K978A, K978Q, K978R, Fig. 2.
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ABCC7 p.Lys978Gln 17582383:77:237
status: NEW84 Similar effects in the presence of Fsk were recorded with cells expressing K978A, K978Q, K978R and K978S mutants CFTR.
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ABCC7 p.Lys978Gln 17582383:84:82
status: NEW96 To investigate the role of the charge of the side chain of K978 in glibenclamide resistance, different amino acid substitutions were examined: K978A, K978Q, K978R.
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ABCC7 p.Lys978Gln 17582383:96:150
status: NEW98 Fig. 4A presents the ratio Iglib/Ifsk, recorded at -100 mV, with 100 bc;M glibenclamide, for K978A, K978Q, K978R, K978S channels compared to CFTR-wt.
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ABCC7 p.Lys978Gln 17582383:98:103
status: NEW108 neutralizing mutations (K978A, K978Q) rendered channels insensitive to inhibition by glibenclamide as demonstrated by the time course of current recorded at +40 mV (Fig. 4B).
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ABCC7 p.Lys978Gln 17582383:108:31
status: NEW128 (B) Examples of time course of current densities (between +40 mVand -40 mV) from the charge neutralizing mutants CFTR-K978Q (a0;) and CFTR-K978A (ef;) in presence of Fsk 10 bc;M, and Fsk+Glib 100 bc;M, and Fsk+CFTRinh-172 10 bc;M.
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ABCC7 p.Lys978Gln 17582383:128:118
status: NEW138 This was the case for the mutations that neutralized the charge of the side chain of the residue 978 (K978A, K978Q, K978S).
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ABCC7 p.Lys978Gln 17582383:138:109
status: NEW