ABCC7 p.Val171Cys
[switch to full view]Comments [show]
None has been submitted yet.
PMID: 18658148
[PubMed]
He L et al: "Multiple membrane-cytoplasmic domain contacts in the cystic fibrosis transmembrane conductance regulator (CFTR) mediate regulation of channel gating."
No.
Sentence
Comment
116
Based on the latter model, we attempted to detect cross-linking of the residue pairs V171C/E407C and V171C/L408C in the whole protein, but did not observe any indication of cross-linking of the mature band (supplemental Fig. S2A).
X
ABCC7 p.Val171Cys 18658148:116:85
status: NEWX
ABCC7 p.Val171Cys 18658148:116:101
status: NEW119 To test this hypothesis, membrane vesicles were prepared from BHK cells overexpressing Cys-less CFTR containing V171C and L408C.
X
ABCC7 p.Val171Cys 18658148:119:112
status: NEW125 We found similar results for the Cys pair V171C and E407C (supplemental Fig. 2B), but not with a Cys pair introduced at Gln-958 and Leu-1261 (supplemental Fig. S2C), confirming that the faster moving CFTR fragment was indeed due to the cross-linking at the CL1/NBD1 interface.
X
ABCC7 p.Val171Cys 18658148:125:42
status: NEW127 No cross-linking was detected when Cys pairs were introduced at L172C/E543C, T966C/D1341C, V171C/L1261C, or M961C/L408C, which are not predicted to be in association in the structural model (supplemental Fig. S3).
X
ABCC7 p.Val171Cys 18658148:127:91
status: NEW135 Membrane vesicles prepared from BHK cells overexpressing Cys-less CFTR containing the Cys pair V171C and L408C were treated with 20 M MTS cross-linker M8M before limited trypsin digestion with concentrations indicated in the figures.
X
ABCC7 p.Val171Cys 18658148:135:95
status: NEW172 Turning from the effects of cross-linking CL3 to those of its counterpart, CL1 in the N-terminal MSD, its cross-linking to NBD1 i.e. V171C/L408C had essentially no influence on gating (Fig. 6C).
X
ABCC7 p.Val171Cys 18658148:172:133
status: NEW203 A, M961C/L1261C at the CL3/NBD2 interface; B, T966C/E543C at the CL3/NBD1 interface; C, V171C/L408C at the CL1/NBD1 interface; D, L171C/D1341C at the CL1/NBD2 interface.
X
ABCC7 p.Val171Cys 18658148:203:88
status: NEW244 We have verified the latter conformation by introducing Cys pairs in CL1 and different residues in RI (V171C/ E407C, V171C/L408C) and showing that these pairs can be cross-linked by M8M (Fig. 3B).
X
ABCC7 p.Val171Cys 18658148:244:103
status: NEWX
ABCC7 p.Val171Cys 18658148:244:117
status: NEW