ABCC7 p.Glu407Cys
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PMID: 18658148
[PubMed]
He L et al: "Multiple membrane-cytoplasmic domain contacts in the cystic fibrosis transmembrane conductance regulator (CFTR) mediate regulation of channel gating."
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116
Based on the latter model, we attempted to detect cross-linking of the residue pairs V171C/E407C and V171C/L408C in the whole protein, but did not observe any indication of cross-linking of the mature band (supplemental Fig. S2A).
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ABCC7 p.Glu407Cys 18658148:116:91
status: NEW125 We found similar results for the Cys pair V171C and E407C (supplemental Fig. 2B), but not with a Cys pair introduced at Gln-958 and Leu-1261 (supplemental Fig. S2C), confirming that the faster moving CFTR fragment was indeed due to the cross-linking at the CL1/NBD1 interface.
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ABCC7 p.Glu407Cys 18658148:125:52
status: NEW244 We have verified the latter conformation by introducing Cys pairs in CL1 and different residues in RI (V171C/ E407C, V171C/L408C) and showing that these pairs can be cross-linked by M8M (Fig. 3B).
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ABCC7 p.Glu407Cys 18658148:244:110
status: NEW