ABCC7 p.Glu543Cys
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PMID: 18658148
[PubMed]
He L et al: "Multiple membrane-cytoplasmic domain contacts in the cystic fibrosis transmembrane conductance regulator (CFTR) mediate regulation of channel gating."
No.
Sentence
Comment
90
Cys pair cross-linking experiments showed that indeed E543C could be cross-linked with both T966C (CL3) and T1057C (CL4, Fig. 2B), while D1341C was in close contact with both L172C (CL1) and N268C (CL2, Fig. 2C).
X
ABCC7 p.Glu543Cys 18658148:90:54
status: NEW101 B, T966C/E543C at the CL3/NBD1 interface and T1057C/E543C at the CL4/NBD1 interface at the X-loop of NBD1.
X
ABCC7 p.Glu543Cys 18658148:101:9
status: NEWX
ABCC7 p.Glu543Cys 18658148:101:52
status: NEW127 No cross-linking was detected when Cys pairs were introduced at L172C/E543C, T966C/D1341C, V171C/L1261C, or M961C/L408C, which are not predicted to be in association in the structural model (supplemental Fig. S3).
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ABCC7 p.Glu543Cys 18658148:127:70
status: NEW142 To determine whether the interfacial interaction of Glu-543 with CL3 and CL4 was indeed affected by PKA phosphorylation, membrane vesicles from HEK cells overexpressing Cys-less CFTR with Cys pairs E543C/T966C and E543C/T1057C were pretreated with PKA in the presence of ATP and Mg2ϩ before cross-linking with various MTS reagents.
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ABCC7 p.Glu543Cys 18658148:142:198
status: NEWX
ABCC7 p.Glu543Cys 18658148:142:214
status: NEW143 As shown in Fig. 4A, similar to the experiments with whole cells in membranes not treated with PKA, the Cys pairs E543C/T966C and E543C/T1057C were cross-linked by all the MTS reagents tested.
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ABCC7 p.Glu543Cys 18658148:143:114
status: NEWX
ABCC7 p.Glu543Cys 18658148:143:130
status: NEW159 Membrane vesicles prepared from HEK cells transiently transfected with Cys pairs introduced at E543C with T966C (CL3) and T1064C (CL4) were pretreated with PKA catalytic subunit in the presence of ATP before incubating with 20 M MTS reagents.
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ABCC7 p.Glu543Cys 18658148:159:95
status: NEW170 In contrast, the cross-linking between residues T996C and E543C at the CL3/NBD1 interface rapidly and reversibly arrested channel gating (Fig. 6B) exactly as we had observed previously with the CL4/NBD1 interface (19).
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ABCC7 p.Glu543Cys 18658148:170:58
status: NEW203 A, M961C/L1261C at the CL3/NBD2 interface; B, T966C/E543C at the CL3/NBD1 interface; C, V171C/L408C at the CL1/NBD1 interface; D, L171C/D1341C at the CL1/NBD2 interface.
X
ABCC7 p.Glu543Cys 18658148:203:52
status: NEW