ABCC7 p.Asn268Cys
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PMID: 18658148
[PubMed]
He L et al: "Multiple membrane-cytoplasmic domain contacts in the cystic fibrosis transmembrane conductance regulator (CFTR) mediate regulation of channel gating."
No.
Sentence
Comment
84
The successful cross-linking of the residue pairs N268C (CL2) and F1294C (NBD2) by the MTS reagents of various spacer arm lengths confirmed this contact (Fig. 2A).
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ABCC7 p.Asn268Cys 18658148:84:50
status: NEW90 Cys pair cross-linking experiments showed that indeed E543C could be cross-linked with both T966C (CL3) and T1057C (CL4, Fig. 2B), while D1341C was in close contact with both L172C (CL1) and N268C (CL2, Fig. 2C).
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ABCC7 p.Asn268Cys 18658148:90:191
status: NEW100 A, 276C/Y1307C and N268C/F1292C at the CL2/NBD2 interface involving an aromatic cluster and the Q-loop.
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ABCC7 p.Asn268Cys 18658148:100:19
status: NEW102 C, L172C/D1341C at the CL1/NBD2 interface and N268C/D1341C at the CL2/NBD2 interface at the X-loop of NBD2.
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ABCC7 p.Asn268Cys 18658148:102:46
status: NEW150 As shown in Fig. 5, treatment of the cells with PKA stimulation mixture before and during cross-linking had no effect on cross-linking between either M498C/G1061C (Fig. 5A, left panel) or N268C/F1294C (Fig. 5B, left panel), although phosphorylation-sensitive CFTR antibody mAb 450 confirmed the phosphorylation of CFTR (Fig. 5, A and B, right panels).
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ABCC7 p.Asn268Cys 18658148:150:188
status: NEW166 Cys pairs were introduced at: A interface between NBD1 Q-loop and CL4 (M498C/G1061C); B, interface between NBD2 Q-loop and CL2 (N268C/ F1294C); or C, both Q-loops (W496C and K1292C).
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ABCC7 p.Asn268Cys 18658148:166:128
status: NEW