ABCMdb

ABCC7 p.Arg117Glu

None has been submitted yet.

Publications

PMID: 18449561 [PubMed] Zhou JJ et al: "Identification of positive charges situated at the outer mouth of the CFTR chloride channel pore."

No. Sentence Comment

60 As shown in Fig. 2, similar inward rectification is observed in the charge-reversing mutants R104E, R117E, and K335E and to a much lesser extent, R1128E, under symmetrical ionic conditions in excised membrane patches. Login to comment
67 In contrast to the linear I-V relationship seen in wild-type CFTR with symmetrical 154 mM Cl- solutions, mutants R104E, R117E, and K335E showed clear inward rectification. Login to comment
73 As shown in Fig. 3b, macroscopic current rectification was indeed sensitive to symmetrical Cl-concentration in R104E, R117E, and K335E, being more pronounced at low Cl-concentration. Login to comment
79 However, current amplitude was significantly reduced in R104E, R117E, and K335E (Fig. 4a, b). Login to comment
91 All mutants depicted (R104Q, R117Q, K335A, R1128Q, R104E, R117E, K335E, R1128E) showed rectification ratios significantly different from wild type (asterisk P<0.05). Login to comment
92 b The degree of rectification in R104E, R117E, and K335E is dependent on the Cl-concentration. Login to comment
95 Mean of data from four to six patches in both a and b side chains bearing different charges (Fig. 5b), with the possible exception that R117C modified by MTSES did not show the same degree of inward rectification seen in R117E. Login to comment
104 In fact, at both sites, pCMBS had a greater effect on the rectification ratio than MTSES (P<0.05), making them closer to results obtained with R104E and R117E. Login to comment
116 All mutants depicted (R104Q, R117Q, K335A, R104E, R117E, K335E) significantly different from wild type (asterisk P<0.05). Login to comment
137 The charge on the side chain is assumed to be +1 (MTSET, WT), 0 (R104/ 117C, R104/117Q), or -1 (MTSES, R104/R117E). Login to comment

PMID: 25024266 [PubMed] Cui G et al: "Three charged amino acids in extracellular loop 1 are involved in maintaining the outer pore architecture of CFTR."

No. Sentence Comment

118 Fig. S6 shows representative I-V plots of double mutants R104E/ E116R- and R117E/E1126R-CFTR and their rectification ratio. Login to comment
128 No current was detected in either inside-out patch or TEVC recording from oocytes expressing R117E-CFTR; therefore, it was not studied further. Login to comment
204 To test this hypothesis, we first made the mutants E116R/R117E-, D110R/R117E-, and D110R/E116R/R117E-CFTR and studied their single-channel properties. Login to comment
224 Tab l e 1 Reversal potentials of WT-CFTR and mutants in ND96 bath solution CFTR n Vrev mV WT 14 &#e032;27.75 &#b1; 0.78 R334A 6 &#e032;12.15 &#b1; 1.64a R117A 6 &#e032;22.51 &#b1; 0.85a E116R 5 &#e032;21.45 &#b1; 1.14a K114D 5 &#e032;24.68 &#b1; 3.22 D110R 5 &#e032;27.64 &#b1; 3.29 R104E 5 &#e032;21.15 &#b1; 1.08a R899C 4 &#e032;25.30 &#b1; 3.94 D891C 6 &#e032;25.81 &#b1; 2.44 K892E 5 &#e032;23.70 &#b1; 3.62 E1124R 5 &#e032;18.32 &#b1; 0.43a E1126R 5 &#e032;20.67 &#b1; 3.16b R117E/E1126R 6 &#e032;23.06 &#b1; 1.37b R104E/E116R 6 &#e032;27.17 &#b1; 1.08 Values are mean &#b1; SEM. Login to comment
271 (A) Representative single-channel currents of R117E/ E116R-, R117E/D110R-, and R117E/E116R/ D110R-CFTR and corresponding all-points amplitude histograms recorded under the same conditions as Fig. 2 A. Login to comment
274 (C) Mean single-channel amplitudes of WT-, R117E/ E116R-, R117E/D110R-, and R117E/E116R/ D110R-CFTR. Login to comment
380 Sample current traces for both E1126R- and R117E/ E1126R-CFTR are shown in Fig. 12 A. Login to comment
381 As mentioned previously, we did not detect current from R117E-CFTR We also tested the possibility that E116 could form an open state salt bridge with K892. Login to comment
403 We also compared the fractional burst duration represented by s1, s2, and f states for E1126R- and R117E/E1126R-CFTR and found that E1126R exhibited a significantly higher fraction of both s1 and s2 states than WT, whereas the distribution for double mutant R117E/E1126R-CFTR was similar to WT-CFTR, exhibiting mainly the f state (a nearly complete rescue; Fig. 12 D). Login to comment
406 R117E/E1126R-CFTR opened to a full open state much more often compared with R117A-CFTR (Fig. 2). Login to comment
407 Mean burst duration for both E1126R- and R117E/ E1126R-CFTR are summarized in Fig. 12 B. Login to comment
408 The mean burst duration of R117E/E1126R-CFTR was significantly longer than that of R117A- and R117C-CFTR. Login to comment
410 (A) Representative single-channel current traces of E1126R-, R117E/E1126R-, R117C-, and R117C/E1126C-CFTR recorded under the same experimental conditions as Fig. 2 and their all-points amplitude histograms (right). Login to comment
412 #, P < 0.01 indicates a significant difference between WTand R117C-CFTR; **, P < 0.01 indicates a significant difference between WTand E1126R-CFTR, between R117C and R117E/E1126R-CFTR, and between R117C and R117C/E1126C. Login to comment
413 n.d., no current detected for R117E-CFTR in Xenopus oocytes. Login to comment
417 (D) Mean fraction of open burst duration is plotted for each open conductance state of E1126R- and R117E/E1126R-CFTR. Login to comment