ABCC7 p.Asp565Ala
[switch to full view]Comments [show]
None has been submitted yet.
PMID: 18361776
[PubMed]
Loo TW et al: "Correctors promote folding of the CFTR in the endoplasmic reticulum."
No.
Sentence
Comment
49
The COPII exit motif (Y563 KDAD567 ) was disrupted by introducing the Y563N or D565A/D567A changes into wild-type CFTR [21] or into the double-cysteine mutants.
X
ABCC7 p.Asp565Ala 18361776:49:79
status: NEW63 Cell-surface labelling of CFTR Wild-type CFTR or COPII exit motif mutant Y563N or D565A/ D567A was transiently expressed in HEK-293 cells.
X
ABCC7 p.Asp565Ala 18361776:63:82
status: NEW145 Tyr563 , Asp565 and Asp567 in the exit motif are evolutionarily Figure 5 Effect of COPII mutations on cross-linking of cysteine mutants (A) Whole-cell SDS extracts of HEK-293 cells expressing wild-type CFTR and wild-type CFTR containing Y563N or D565A/D567A mutation were subjected to immunoblot analysis.
X
ABCC7 p.Asp565Ala 18361776:145:249
status: NEW146 (B) HEK293 cells expressing wild-type CFTR or wild-type CFTR containing Y563N or D565A/D567A mutant were surface-labelled with sulfo-NHS-SS-biotin.
X
ABCC7 p.Asp565Ala 18361776:146:81
status: NEW158 Accordingly, we introduced the Y563N or D565A/D567A mutations in the COPII exit motif of wild-type CFTR.
X
ABCC7 p.Asp565Ala 18361776:158:40
status: NEW161 Cells expressing wild-type CFTR or Y563N or D565A/D567A mutant were treated with sulfo-NHS-SS-biotin, and then lysed with detergent.
X
ABCC7 p.Asp565Ala 18361776:161:44
status: NEW
PMID: 23890012
[PubMed]
Farinha CM et al: "Revertants, low temperature, and correctors reveal the mechanism of F508del-CFTR rescue by VX-809 and suggest multiple agents for full correction."
No.
Sentence
Comment
174
(C) BHK cell lines stably expressing WT-CFTR or bearing the DD/AA mutations (D565A, D567A) alone or in cis with 4RK were cultured at 37 C, incubated at 26 C for 48 hr or for 24 hr with VRT-325, Corr-4a, or VX-809, as indicated. CFTR protein was analyzed by WB. Data are representative of n = 6 experiments.
X
ABCC7 p.Asp565Ala 23890012:174:77
status: NEW231 EXPERIMENTAL PROCEDURES Cells and Culture Conditions BHK cell lines expressing F508del-4RK (R29K/R516K/R555K/R716K)-, F508del-G550E-, F508del-R1070W-, F508del-V510D-, F508del-R555K-, F508del-V510D/G550E-, F508del-G550E/R1070W-, DAA (D567A)-, 4RK- DAA-, DD/AA (D565A, D567A)-, 4RK-DD/AA-, and R560T-CFTR were produced and cultured as previously described (Roxo-Rosa et al., 2006).
X
ABCC7 p.Asp565Ala 23890012:231:260
status: NEW