ABCMdb

ABCC7 p.Met498Cys

None has been submitted yet.

Publications

PMID: 18305154 [PubMed] Serohijos AW et al: "Phenylalanine-508 mediates a cytoplasmic-membrane domain contact in the CFTR 3D structure crucial to assembly and channel function."

No. Sentence Comment

81 In addition to the Phe-508-containing NBD1 surface patch, residues in other regions of the domain also interact with CL4 residues as evidenced by cross-linking of Cys pairs involving amino acids closer to the Q loop (Gln-493), including W496C/T1064C and M498C/L1065C as well as nearer the Walker B motif (Asp-572) such as K564C/G1069C (Fig. 3A and SI Fig. 8). Login to comment
97 Phe-508 participates in an apparent aromatic cluster with residues from CL4(seealsoSIFig.10).CL4alsointeractswithotherregionsinNBD1assuggested by cross-linking of residues close to the Q loop (W496C/T1064C and M498C/ L1065C) and a residue near the Walker B motif (K564C/G1069C). Login to comment

PMID: 18658148 [PubMed] He L et al: "Multiple membrane-cytoplasmic domain contacts in the cystic fibrosis transmembrane conductance regulator (CFTR) mediate regulation of channel gating."

No. Sentence Comment

80 In agreement with what is predicted by our model, we were able to cross-link residue pairs W496C/T1064C, M498C/L1065C (19), which proves that indeed CL4 also interacts with NBD1 through the so called Q-loop (Q493). Login to comment
150 As shown in Fig. 5, treatment of the cells with PKA stimulation mixture before and during cross-linking had no effect on cross-linking between either M498C/G1061C (Fig. 5A, left panel) or N268C/F1294C (Fig. 5B, left panel), although phosphorylation-sensitive CFTR antibody mAb 450 confirmed the phosphorylation of CFTR (Fig. 5, A and B, right panels). Login to comment
166 Cys pairs were introduced at: A interface between NBD1 Q-loop and CL4 (M498C/G1061C); B, interface between NBD2 Q-loop and CL2 (N268C/ F1294C); or C, both Q-loops (W496C and K1292C). Login to comment