ABCC7 p.Arg1030Gln
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PMID: 17043152
[PubMed]
Aubin CN et al: "Positive charges at the intracellular mouth of the pore regulate anion conduction in the CFTR chloride channel."
No.
Sentence
Comment
131
However, both R134Q and R1030Q did yield functional channels (Fig. 10).
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ABCC7 p.Arg1030Gln 17043152:131:24
status: NEW142 R1030Q, like wild type, was associated with an almost linear I-V relationship (Fig. 10, A and B).
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ABCC7 p.Arg1030Gln 17043152:142:0
status: NEW145 R1030Q single channel currents were readily observed (Fig. 10 C), and these channels showed a slightly, but significantly, reduced single channel conductance (Fig. 10 D) of 4.70 ± 0.04 pS (n = 4) compared with 5.39 ± 0.06 pS (n = 5) for wild type (P < 0.0001, two-tailed t test).
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ABCC7 p.Arg1030Gln 17043152:145:0
status: NEW172 (A) Example leak-subtracted macroscopic I-V relationships from R134Q and R1030Q-CFTR in inside-out membrane patches with symmetrical 154 mM Cl- solu- tions, after maximal channel activation.
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ABCC7 p.Arg1030Gln 17043152:172:73
status: NEW179 (D) Mean single channel current-voltage relationships for wild type (filled circles) and R1030Q (open circles).
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ABCC7 p.Arg1030Gln 17043152:179:89
status: NEW207 R134Q and R1030Q do not appear consistent with loss of an intracellular surface charge.
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ABCC7 p.Arg1030Gln 17043152:207:10
status: NEW210 R1030Q did not alter macroscopic current rectification (Fig. 10 B) but was associated with a small decrease in unitary conductance (Fig. 10 D), suggesting that while this residue may play a role in determining pore properties, it does not act as an important inner surface charge.
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ABCC7 p.Arg1030Gln 17043152:210:0
status: NEW