ABCMdb

ABCC7 p.Arg134Gln

None has been submitted yet.

Publications

PMID: 17043152 [PubMed] Aubin CN et al: "Positive charges at the intracellular mouth of the pore regulate anion conduction in the CFTR chloride channel."

No. Sentence Comment

131 However, both R134Q and R1030Q did yield functional channels (Fig. 10). Login to comment
132 R134Q Figure 7. Modification of CFTR by intracellular MTSES. Login to comment
143 Measurable single channel activity was not observed after R134Q expression; however, Fig. 10 C shows an example of a very small apparent single channel opening frequently observed in a patch excised from an R134Q-expressing cell. Login to comment
144 This tiny current suggests that R134Q has an extremely small unitary conductance below the accurate resolution of single channel recording. Login to comment
172 (A) Example leak-subtracted macroscopic I-V relationships from R134Q and R1030Q-CFTR in inside-out membrane patches with symmetrical 154 mM Cl- solu- tions, after maximal channel activation. Login to comment
174 * indicates a significant difference from wild type at the same Cl-concentration (P < 10-4, two-tailed t test), † indicates a significant difference from R134Q at 154 mM Cl- (P < 0.005, two-tailed t test). Login to comment
177 For R134Q, the tiny current associated with a putative channel opening is identified by a line above it. Login to comment
207 R134Q and R1030Q do not appear consistent with loss of an intracellular surface charge. Login to comment
208 R134Q is associated with outward rectification (Fig. 10, A and B); however, this effect shows a complex Cl-dependence that, unlike that of R303E, R303Q, and R352Q (Fig. 3 B), is not consistent with a simple surface charge effect. Furthermore, R134Q shows an extremely small apparent unitary conductance (Fig. 10 C). Login to comment