ABCMdb

ABCC7 p.Cys592Leu

None has been submitted yet.

Publications

PMID: 17036051 [PubMed] Mense M et al: "In vivo phosphorylation of CFTR promotes formation of a nucleotide-binding domain heterodimer."

No. Sentence Comment

26 Nor could C590 and C592 be replaced by alanine, threonine or phenylalanine (Figure 2B), but function was similar to that of the 16CS background when they were replaced by leucines (16CS þ C590L/C592L; Figure 2A and B) or valines (16CS þ C590V/C592V; Figure 2B). Login to comment
29 B Resting Stimulated 16CS+C590V/C592V 16CS+C590L/C592L 16CS+C590F/C592F 16CS+C590T/C592T 16CS+C590S/C592S 16CS+C590/C592 16CS+C590A/C592A A 200 s 5 µA WT CFTR Cys-free CFTR 16CS+C590L/C592L 0 25 50 75 100 125 150 175 WT CFTR Whole-oocyte conductance (µS) 16CS+C590V/C592V 16CS+C590/C592 C (2.5 ng cRNA) (20 ng cRNA) 250 160 105 75 kD cRNA (ng) 0.25 20 2.5 20 WT CFTR 16CS+ C590V/C592V 1 2 3 4 Mature Core D Uninjected oocyte 40 µM forskolin 40 µM forskolin 40 µM forskolin Washout Washout Washout Figure 2 Expression and function of cysteine-deficient CFTR channels in Xenopus oocytes. Login to comment
30 (A) Two-microelectrode voltage-clamp current recordings from uninjected oocyte and oocytes expressing WT CFTR (2.5 ng cRNA) or HA-tagged Cys-free CFTR 16CS þ C590L/C592L (20 ng cRNA); vertical current deflections monitor conductance, which was transiently increased by brief exposure to 40 mM forskolin (between arrows). Login to comment
199 For recording macroscopic currents of split CFTR channels in excised patches (Figure 10), oocytes were Table I Forward primers for site-directed mutagenesis PCR C76S 50 -GCCCTTCGGCGATcgTTTTTCTGGAG-30 C276S 50 -CTGTTAAGGCCTACTcCTGGGAAGAAGC-30 C832S 50 -CGAAGAAGACCTTAAGGAGTcCTTTTTTGATGATATGGAGAGC-30 EagI site 50 -GGTAAAATTAAGCACAGcGGccGAATTTCATTCTGTTCTC-30 HA epitope 50 -CGGGCCGCCATGtAcccatAcGACGttccgGAttAcgcaAGGTCGCCTCTGG-30 CFTR 16CS C590A/C592A 50 -GGAGATCTTCGAGAGCgCTGTCgCTAAACTGATGGC-30 CFTR 16CS C590F/C592F 50 -GGAGATCTTCGAGAGCTtTGTCTtTAAACTGATGGC-30 CFTR 16CS C590L/C592L 50 -GGAGATCTTCGAGAGCctTGTCctTAAACTGATGGC-30 CFTR 16CS C590T/C592T 50 -GGAGATCTTCGAGAGCaCTGTCaCTAAACTGATGGC-30 CFTR 16CS C590V/C592V 50 -GGAGATCTTCGAGAGCgtcGTCgtTAAACTGATGGC-30 S434C 50 -CCTCTTCTTCAGTAATTTCTgtCTaCTTGGTACTCCTGTC-30 S459C 50 -GTTGGCGGTTGCTGGATgCACTGGAGCAGGCAAG-3 A462C 50 -GCTGGATCCACTGGGtgcGGCAAGACTTCACTTC-30 L549C 50 -GGTGGAATCACACtatGcGGAGGTCAACGAGCACG-30 S605C 50 -GGATTTTGGTCACaTgTAAAATGGAAC-30 S1248C 50 -CCTCTTGGGAAGAACCGGtTgtGGGAAGAGTAC-30 D1336C 50 -GTTTCCTGGGAAGCTTtgCTTTGTCCTTGTGG-30 L1346C 50 -GGATGGGGGCTCTGTCTgtAGTCATGGCCACAAGC-30 A1374C 50 -GATGAACCAAGCtgTCATTTAGATCC-30 V1379C 50 -GCTCATTTAGATCCgtgcACATACCAAATAATTCG-30 The underlined bases are the codons for the introduced serines, cysteines or other residues; lowercase letters mark base changes from the original sequence, including those for introducing diagnostic restriction endonuclease sites. Login to comment

PMID: 17911111 [PubMed] Wang Y et al: "Correctors promote maturation of cystic fibrosis transmembrane conductance regulator (CFTR)-processing mutants by binding to the protein."

No. Sentence Comment

23 Cys-less CFTR was constructed by replacing Cys590 and Cys592 with leucine (9) and the other 16 endogenous cysteines at positions 76, 126, 225, 276, 343, 491, 524, 657, 832, 866, 1344, 1355, 1395, 1400, 1410, and 1458 with alanine. Login to comment

PMID: 18361776 [PubMed] Loo TW et al: "Correctors promote folding of the CFTR in the endoplasmic reticulum."

No. Sentence Comment

46 A cysteine-less CFTR was constructed by replacing Cys590 and Cys592 with leucine and by changing all other endogenous cysteine residues to alanine [23]. Login to comment
92 Cysteine-less CFTR, in which Cys590 and Cys592 were replaced with leucine and the remaining cysteine residues were changed to alanine [23], did not mature at 37◦ C unless Val510 (in NBD1) was changed to alanine [17]. Login to comment

PMID: 19754156 [PubMed] Alexander C et al: "Cystic fibrosis transmembrane conductance regulator: using differential reactivity toward channel-permeant and channel-impermeant thiol-reactive probes to test a molecular model for the pore."

No. Sentence Comment

42 The Cys-less CFTR construct (C76S, C126S, C225S, C276S, C343S, C491S, C524S, C590L, C592L, C657S, C832S, C866S, C1344S, C1355S, C1395S, C1400S, C1410S, C1458S) was a gift from Drs. Martin Mense and David Gadsby and was used in their pGEMHE vector previously described (13). Login to comment

PMID: 16766608 [PubMed] Serrano JR et al: "CFTR: Ligand exchange between a permeant anion ([Au(CN)2]-) and an engineered cysteine (T338C) blocks the pore."

No. Sentence Comment

23 MATERIALS AND METHODS Mutagenesis and in vitro transcription The Cys-less CFTR construct (C76S, C126S, C225S, C276S, C343S, C491S, C524S, C590L, C592L, C657S, C832S, C866S, C1344S, C1355S, C1395S, C1400S, C1410S, C1458S) was a gift from Drs. Martin Mense and Submitted December 28, 2005, and accepted for publication May 19, 2006. Login to comment

PMID: 23709221 [PubMed] Cui G et al: "Two salt bridges differentially contribute to the maintenance of cystic fibrosis transmembrane conductance regulator (CFTR) channel function."

No. Sentence Comment

49 All cRNAs for single channel recording were prepared from constructs encoding WT-CFTR or Cys-less CFTR (16C 3 S, C590L, C592L) in the pGEMHE vector, which was kindly provided by Dr. D. Gadsby (Rockefeller University) as reported previously (21). Login to comment