ABCC7 p.Arg555Gln
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PMID: 15729345
[PubMed]
Vergani P et al: "CFTR channel opening by ATP-driven tight dimerization of its nucleotide-binding domains."
No.
Sentence
Comment
85
c, Mutations at Arg 555 in NBD1 'tail` affect mean open burst (R555Q) and closed interburst (R555K) dwell times.
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ABCC7 p.Arg555Gln 15729345:85:63
status: NEW86 R555Q opened with rates comparable to WT (R555Q, tib ¼ 2.84 ^ 0.53 s (15)) despite expected loss of the interfacial hydrogen bond, suggesting possible compensation by other factors, for example the removal of repulsive electrostatic forces.
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ABCC7 p.Arg555Gln 15729345:86:0
status: NEWX
ABCC7 p.Arg555Gln 15729345:86:42
status: NEW95 We first mutated Arg 555, and found that the charge-removing mutation R555Q slowed channel closing (mean open burst duration was 3.20 ^ 0.35 s (n ¼ 18) for R555Q, compared with 0.43 ^ 0.02 s (n ¼ 32) for WT; Fig. 2c), consistent with Arg 555 being part of the composite NBD2 catalytic site, where the ATP hydrolysis that times closing of WT channels occurs.
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ABCC7 p.Arg555Gln 15729345:95:70
status: NEWX
ABCC7 p.Arg555Gln 15729345:95:161
status: NEW