ABCC7 p.Ser589Asn

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PMID: 12939655 [PubMed] Perri F et al: "Mutation analysis of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, the cationic trypsinogen (PRSS1) gene, and the serine protease inhibitor, Kazal type 1 (SPINK1) gene in patients with alcoholic chronic pancreatitis."
No. Sentence Comment
34 Other seven mutations, frequently observed in Italian CF patients (Q552X; 711+5G4A; 2790-2A4G; S589N; T338I; 1898+3A4G, and 1717-8G4A), were examined by sequencing.
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ABCC7 p.Ser589Asn 12939655:34:95
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PMID: 16379540 [PubMed] Stanziale P et al: "Indirect CFTR mutation identification by PCR/OLA anomalous electropherograms."
No. Sentence Comment
2 Here we report the description of five cases of anomalous electropherograms obtained after PCR/OLA analysis, that led to the identification, in the homozygous state, of two point mutations (D110H and S589N) not included in the assay test panel, a large gene deletion (CFTRdel14b_17b), and an exonic polymorphism (c.4002A Ͼ G).
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ABCC7 p.Ser589Asn 16379540:2:200
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46 B: Sequencing analysis of exon 12 in case 2 and wild-type control sample. The arrow indicates the nucleotide change leading to the S589N mutation.
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ABCC7 p.Ser589Asn 16379540:46:131
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50 FREQUENCY DISTRIBUTION OF CFTR MUTATIONS IDENTIFIED IN 116 PATIENTS WITH CYSTIC FIBROSIS ORIGINATING FROM CENTRAL-SOUTHERN ITALY Mutations Allele frequency (%) F508del 47.41 G542X 9.48 N1303K 5.60 G85E 5.17 2789ϩ5GϾA 1.29 621ϩ1G-ϾT 1.29 R347P 1.29 R553X 1.29 S589N 1.29 W1282X 1.29 CFTRdele14b-17b 0.86 1717-1G-ϾA 0.43 2183 AA-ϾG 0.43 R1162X 0.43 R334W 0.43 711ϩ5G-ϾA 0.43 3849ϩ1OKbC-ϾT 0.43 Unidentified 21.12 A B C D GTTG-3Ј), 14bF (5Ј-GGGAGGAATAGGTGAAGAT-3Ј) and 14bR (5Ј-AATCCACTATGTTTGTATGTA-3Ј), 17bF (5Ј-AA- TGACATTTGTGATATGAT-3Ј) and 17bR (5Ј-ACTTTAG- CTAAGCATTTAAG-3Ј), respectively.
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ABCC7 p.Ser589Asn 16379540:50:283
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66 Sequencing of exon 12 and flanking intronic regions revealed the presence of the nucleotide transition G to A at position c.1898 in the homozygous state generating the substitution of asparagine for serine at codon 589 (S589N) (Fig. 2B).
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ABCC7 p.Ser589Asn 16379540:66:184
status: NEW
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ABCC7 p.Ser589Asn 16379540:66:220
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118 In addition, because the D110H and S589N changes, similar to CFTRdel14b_17b, are rare mutations (CF Genetic Analysis Consortium: www.genet.sickkids.on.ca/CFTR) and the c.4002A Ͼ G allelic frequency is 1.32%, as resulted by the analysis performed in 113 control individuals from central-southern Italy (data not shown), it seems to be reasonable to postulate consanguinity in all the families.
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ABCC7 p.Ser589Asn 16379540:118:35
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PMID: 17062471 [PubMed] Merelle ME et al: "Extended gene analysis can increase specificity of neonatal screening for cystic fibrosis."
No. Sentence Comment
58 Results Reliability of the extended gene analysis The following mutations were found in the study population: DF508, 3659delC, R553X, S589N, R1070Q and E60X (Table I).
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ABCC7 p.Ser589Asn 17062471:58:134
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59 The 3659delC, R553X, S589N and R1070Q mutations were identified by OLA analysis.
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ABCC7 p.Ser589Asn 17062471:59:21
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94 Genotypes Number DF508/DF508 5 DF508/3659delC 1 DF508/R553X 1 DF508/S589N 1 DF508/R1070Q 1 DF508/E60X 1 DF508/N 8 3659delC/N 1 S589N/N 1 Total 20 N: no CFTR mutation.
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ABCC7 p.Ser589Asn 17062471:94:68
status: NEW
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ABCC7 p.Ser589Asn 17062471:94:127
status: NEW
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