ABCC7 p.Phe446Cys
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PMID: 10893239
[PubMed]
Berger AL et al: "Differences between cystic fibrosis transmembrane conductance regulator and HisP in the interaction with the adenine ring of ATP."
No.
Sentence
Comment
9
Although modification inactivated CFTR-Y1219C more rapidly than wild-type CFTR, and inactivation of CFTR-F446C was nucleotide-dependent; failure of these mutations to alter gating suggested that Tyr1219 and Phe446 were not important for nucleotide binding.
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ABCC7 p.Phe446Cys 10893239:9:105
status: NEW77 When expressed in HeLa cells, CFTR-F429C, F430C, F433C, F446C, Y1219C, and F1232C all generated Cl-channel activity in excised, inside-out patches of membrane.
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ABCC7 p.Phe446Cys 10893239:77:56
status: NEW126 Construct Po WT 0.29 Ϯ 0.04 F429C 0.21 Ϯ 0.03 F430C 0.30 Ϯ 0.03 F433C 0.21 Ϯ 0.03 F446C 0.34 Ϯ 0.06 Y1219C 0.26 Ϯ 0.07 F1232C 0.35 Ϯ 0.02 FIG. 2.
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ABCC7 p.Phe446Cys 10893239:126:106
status: NEW142 CFTR variant k M -1 s-1 Wild-type 23.1 Ϯ 6.4 F429C 27.0 Ϯ 16.7 F430C 34.6 Ϯ 22.8 F433C 19.0 Ϯ 5.5 F446C 36.1 Ϯ 15.8 Y1219C 75.5 Ϯ 20.1* F1232C 68.5 Ϯ 19.6 not interfere with the ability of MTSET to inactivate CFTR-Y1219C.
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ABCC7 p.Phe446Cys 10893239:142:122
status: NEW151 In contrast to the other channels, CFTR-F446C showed a more striking inhibition by MTSET in the absence than in the presence of ATP (compare Fig. 4, A and B, with Fig. 3) that was not explained by the rate of inhibition observed in the presence of ATP (Table II).
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ABCC7 p.Phe446Cys 10893239:151:40
status: NEW155 Reaction of CFTR-F446C with MTSET for 60 s in the presence of 1 mM ADP preserved almost all CFTR activity (Fig. 6).
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ABCC7 p.Phe446Cys 10893239:155:17
status: NEW191 CFTR-F446C was the only channel in which nucleotide altered the inactivation rate.
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ABCC7 p.Phe446Cys 10893239:191:5
status: NEW