ABCMdb

ABCC7 p.Asp651*

None has been submitted yet.

Publications

PMID: 10220462 [PubMed] Schreiber R et al: "The first-nucleotide binding domain of the cystic-fibrosis transmembrane conductance regulator is important for inhibition of the epithelial Na+ channel."

No. Sentence Comment

45 (v) The N-terminal CFTR truncation including the N terminus, the first six transmembrane helices, and an extended NBF-1, according to a remodeled NBF-1 (ref. 17; D651X), was generated with a SpeI͞BamHI CFTR fragment (M1-A457), and the PCR product was generated with 5Ј-GAGGAGGGATTTGGG- GAAT-3Ј (s) and 5Ј-TTAGAAAGAATCACATC- CCATGA-3Ј (as). Login to comment
97 Several truncated versions of CFTR were generated to identify CFTR domains essential to the down-regulation of ENaC: (i) the central cytosolic part of CFTR including two transmembrane helices on each site (M284͞I942X); (ii) NBF-1 (W401M͞D651X); (iii) the N-terminal half of CFTR without NBF-1 (E402X); (iv) the N-terminal half of CFTR including NBF-1 (C590X); (v) the N-terminal half of CFTR including an additional stretch of 61 amino acids, as described in a recent study (ref. 17; D651X); (vi) the N-terminal half of CFTR including NBF-1 and the R domain (E831X); and (vii) the C-terminal half including the R domain but not NBF-1 (M595-C). Login to comment

PMID: 12606488 [PubMed] Cheung KH et al: "Synergistic effects of cystic fibrosis transmembrane conductance regulator and aquaporin-9 in the rat epididymis."

No. Sentence Comment

44 Domain amplified Primer sequencea Corresponding size (kilobases) M595-C (C-terminal half of CFTR including R domain but no NBD1) E402X (N-terminal half of CFTR but no NBD1) W401M-D651X (NBD1 only) F: 5Ј-TTTGAAAAGTTGTGTCTGT-3Ј R: 5Ј-AGCCTCGAGCACTAGAGC-3Ј F: 5Ј-AGACATCATGCAGAAGTCGCCT-3Ј R: 5Ј-CTCCCAAAATGCTGTTACATT-3Ј F: 5Ј-ACAGCAACCTGGAGGAGGGATTTCAG-3Ј R: 5Ј-CTCGAGCATAAAAGTATCATACCCCAT-3Ј 2.7 1.2 0.8 a F, forward; R, reverse. Login to comment
50 In brief, the truncated forms of CFTR, M595-C (C-terminal half of CFTR including R domain but lacking NBD1), E402X (N-terminal half of CFTR but lacking NBD1), and W401M-D651X (NBD1 only), were PCR amplified by specific primers (Table 1) and cloned into pGEM-T cloning vectors (Promega). Login to comment
130 However, W40M-D651X (a fragment containing NBD1 only) preserved some of the activities of wild-type CFTR in potentiating AQP-9-induced water permeability in the oocytes (Fig. 3). Login to comment

PMID: 10869574 [PubMed] Schreiber R et al: "Aquaporin 3 cloned from Xenopus laevis is regulated by the cystic fibrosis transmembrane conductance regulator."

No. Sentence Comment

46 Oocytes of identical batches were injected with 20 ng cRNA of wild type (wt) CFTR, G551D-CFTR, xAQP3 and truncated forms of CFTR: M595-C (C-terminal half of CFTR including R-domain but not NBD1); C590X (N-terminal half of CFTR including N-terminus, the 'rst six transmembrane helices and NBD1 but not R-domain); M394-K830 (NBD1 and R-domain); W401M-D651X (NBD1 only). Login to comment
139 After stimulation Pgly was enhanced in wtCFTR, C590X and W410M-D651X expressing oocytes but not in oocytes injected with the C-terminal half of CFTR (M595-C). Login to comment