ABCC7 p.Cys590*
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PMID: 10220462
[PubMed]
Schreiber R et al: "The first-nucleotide binding domain of the cystic-fibrosis transmembrane conductance regulator is important for inhibition of the epithelial Na+ channel."
No.
Sentence
Comment
44
5310 terminal CFTR truncation including the N terminus, the first six transmembrane helices, and NBF-1 (C590X) was obtained as a SpeI͞KpnI CFTR cDNA fragment.
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ABCC7 p.Cys590* 10220462:44:105
status: NEW97 Several truncated versions of CFTR were generated to identify CFTR domains essential to the down-regulation of ENaC: (i) the central cytosolic part of CFTR including two transmembrane helices on each site (M284͞I942X); (ii) NBF-1 (W401M͞D651X); (iii) the N-terminal half of CFTR without NBF-1 (E402X); (iv) the N-terminal half of CFTR including NBF-1 (C590X); (v) the N-terminal half of CFTR including an additional stretch of 61 amino acids, as described in a recent study (ref. 17; D651X); (vi) the N-terminal half of CFTR including NBF-1 and the R domain (E831X); and (vii) the C-terminal half including the R domain but not NBF-1 (M595-C).
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ABCC7 p.Cys590* 10220462:97:364
status: NEW117 As shown as an example in Fig. 6A, the N-terminal half of CFTR without the R domain (C590X) is able to inhibit ENaC.
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ABCC7 p.Cys590* 10220462:117:85
status: NEW128 FIG. 6. Time course of the whole-cell conductance measured in oocytes coexpressing C590X and ␣-, beta-, and ␥-ENaC (A) or M595-C and ␣-, beta-, and ␥-ENaC (B).
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ABCC7 p.Cys590* 10220462:128:83
status: NEW129 Amiloride (Amil; 10 mol͞liter) blocked most of the whole-cell conductance when applied under control conditions. In C590X-expressing oocytes, whole-cell conductances were inhibited, and the effect of amiloride was attenuated after stimulation with IBMX and forskolin.
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ABCC7 p.Cys590* 10220462:129:130
status: NEW
PMID: 10869574
[PubMed]
Schreiber R et al: "Aquaporin 3 cloned from Xenopus laevis is regulated by the cystic fibrosis transmembrane conductance regulator."
No.
Sentence
Comment
46
Oocytes of identical batches were injected with 20 ng cRNA of wild type (wt) CFTR, G551D-CFTR, xAQP3 and truncated forms of CFTR: M595-C (C-terminal half of CFTR including R-domain but not NBD1); C590X (N-terminal half of CFTR including N-terminus, the 'rst six transmembrane helices and NBD1 but not R-domain); M394-K830 (NBD1 and R-domain); W401M-D651X (NBD1 only).
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ABCC7 p.Cys590* 10869574:46:196
status: NEW139 After stimulation Pgly was enhanced in wtCFTR, C590X and W410M-D651X expressing oocytes but not in oocytes injected with the C-terminal half of CFTR (M595-C).
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ABCC7 p.Cys590* 10869574:139:47
status: NEW