ABCC7 p.Glu402*
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PMID: 10220462
[PubMed]
Schreiber R et al: "The first-nucleotide binding domain of the cystic-fibrosis transmembrane conductance regulator is important for inhibition of the epithelial Na+ channel."
No.
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Comment
34
(iii) The N-terminal CFTR truncation including the N terminus and the first six transmembrane helices (E402X) was generated with 5Ј-CCC- GAGGTACCATGCAGAGGTC-3Ј (s) and 5Ј-CTACTCGA- GCTACCAGAAGGCTGTTACATTC-3Ј (as).
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ABCC7 p.Glu402* 10220462:34:103
status: NEW97 Several truncated versions of CFTR were generated to identify CFTR domains essential to the down-regulation of ENaC: (i) the central cytosolic part of CFTR including two transmembrane helices on each site (M284͞I942X); (ii) NBF-1 (W401M͞D651X); (iii) the N-terminal half of CFTR without NBF-1 (E402X); (iv) the N-terminal half of CFTR including NBF-1 (C590X); (v) the N-terminal half of CFTR including an additional stretch of 61 amino acids, as described in a recent study (ref. 17; D651X); (vi) the N-terminal half of CFTR including NBF-1 and the R domain (E831X); and (vii) the C-terminal half including the R domain but not NBF-1 (M595-C).
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ABCC7 p.Glu402* 10220462:97:306
status: NEW98 With the exception of E402X, expression of all of these constructs enhanced baseline whole-cell Cl-conductance when compared with water-injected control oocytes (Fig. 4).
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ABCC7 p.Glu402* 10220462:98:22
status: NEW132 However, the results indicate that, except for M595-C and E402X, all CFTR truncations inhibited ENaC conductances slightly (about 25%) but significantly (P Ͻ 0.01) during stimulation.
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ABCC7 p.Glu402* 10220462:132:58
status: NEW133 Because M595-C and E402X are the only CFTR truncations that do not contain NBF-1, these results suggest that CFTR`s ability to inhibit ENaC relies on the presence of an intact NBF-1.
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ABCC7 p.Glu402* 10220462:133:19
status: NEW160 GAmiloride values measured under control conditions (white bars) were significantly smaller after stimulation of the oocytes with IBMX and forskolin (black bars), except for E402X and M959-C. The numbers of experiments are shown in parentheses.
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ABCC7 p.Glu402* 10220462:160:174
status: NEW
PMID: 12606488
[PubMed]
Cheung KH et al: "Synergistic effects of cystic fibrosis transmembrane conductance regulator and aquaporin-9 in the rat epididymis."
No.
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44
Domain amplified Primer sequencea Corresponding size (kilobases) M595-C (C-terminal half of CFTR including R domain but no NBD1) E402X (N-terminal half of CFTR but no NBD1) W401M-D651X (NBD1 only) F: 5Ј-TTTGAAAAGTTGTGTCTGT-3Ј R: 5Ј-AGCCTCGAGCACTAGAGC-3Ј F: 5Ј-AGACATCATGCAGAAGTCGCCT-3Ј R: 5Ј-CTCCCAAAATGCTGTTACATT-3Ј F: 5Ј-ACAGCAACCTGGAGGAGGGATTTCAG-3Ј R: 5Ј-CTCGAGCATAAAAGTATCATACCCCAT-3Ј 2.7 1.2 0.8 a F, forward; R, reverse.
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ABCC7 p.Glu402* 12606488:44:129
status: NEW50 In brief, the truncated forms of CFTR, M595-C (C-terminal half of CFTR including R domain but lacking NBD1), E402X (N-terminal half of CFTR but lacking NBD1), and W401M-D651X (NBD1 only), were PCR amplified by specific primers (Table 1) and cloned into pGEM-T cloning vectors (Promega).
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ABCC7 p.Glu402* 12606488:50:109
status: NEW129 NBD1) nor E402X (C-terminal half of CFTR including R domain but lacking NBD1) potentiated AQP-9.
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ABCC7 p.Glu402* 12606488:129:10
status: NEW