ABCMdb

ABCC1 p.Glu521Asp

None has been submitted yet.

Publications

PMID: 21177244 [PubMed] Iram SH et al: "Expression and function of human MRP1 (ABCC1) is dependent on amino acids in cytoplasmic loop 5 and its interface with nucleotide binding domain 2."

No. Sentence Comment

141 Expression and Transport Activity of "Same Charge" Mutants of Lys513 , Lys516 , Glu521 , and Glu535 -To determine whether it was the charge or another property of the amino acid at positions 513, 516, 521, and 535 that was critical for efficient plasma membrane expression of MRP1, same charge mutants (K513R, K516R, E521D, and E535D) were created and again expressed in HEK cells. Login to comment
142 At 37 °C, levels of the K513R mutant were comparable with wild-type MRP1 levels, whereas levels of the K516R, E521D, and E535D mutants were somewhat reduced (20-40%) (Fig. 4A). Login to comment
148 A and B, representative immunoblots of whole cell lysates (WCL) (10 ␮g of protein/lane) (A) and membrane vesicles (1 ␮g of protein/lane) (B) prepared from HEK293T cells transfected with wild-type (WT-MRP1) and mutant (K513R, K516R, E521D, and E535D) MRP1 cDNA expression vectors. Untransfected cells were used as a negative control. MRP1 levels were detected with mAb QCRL-1, and the relative protein expression levels, estimated by densitometry, are shown below the blots. Login to comment