ABCC1 p.His1364Ala
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PMID: 21177244
[PubMed]
Iram SH et al: "Expression and function of human MRP1 (ABCC1) is dependent on amino acids in cytoplasmic loop 5 and its interface with nucleotide binding domain 2."
No.
Sentence
Comment
51
To engineer mutations of His1364 and Arg1367 in NBD2, a 0.8-kb EcoRI/KpnI fragment of MRP1 in pBluescriptSK(ϩ) was used as the template with the following mutagenic primers (substituted nucleotides are underlined): H1364A, 5Ј-GAT CGG CCT GGC CGA CCT CCG C-3Ј; and R1367A, 5Ј-CTG CAC GAC CTC GCC TTC AAG ATC AC-3Ј.
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ABCC1 p.His1364Ala 21177244:51:221
status: NEW158 To test this hypothesis, mutants H1049A, H1364A, and R1367A were generated, and the levels of the mutant proteins in HEK293T cells determined as described above.
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ABCC1 p.His1364Ala 21177244:158:41
status: NEW160 The levels of the NBD2 mutant H1364A and the CL6 mutant H1049A were also reduced by Ͼ50%.
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ABCC1 p.His1364Ala 21177244:160:30
status: NEW178 Homology models and levels of MRP1 mutants H1049A, H1364A, and R1367A.
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ABCC1 p.His1364Ala 21177244:178:51
status: NEW227 This hypothesis was supported by our observations that MRP1 expression is retained when the three CL5 residues are replaced with same charge amino acids but is lost or reduced when either the NBD2 residue Arg1367 or His1364 is replaced with Ala.
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ABCC1 p.His1364Ala 21177244:227:216
status: NEW