ABCC1 p.Arg1367Ala
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PMID: 21177244
[PubMed]
Iram SH et al: "Expression and function of human MRP1 (ABCC1) is dependent on amino acids in cytoplasmic loop 5 and its interface with nucleotide binding domain 2."
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Sentence
Comment
51
To engineer mutations of His1364 and Arg1367 in NBD2, a 0.8-kb EcoRI/KpnI fragment of MRP1 in pBluescriptSK(ϩ) was used as the template with the following mutagenic primers (substituted nucleotides are underlined): H1364A, 5Ј-GAT CGG CCT GGC CGA CCT CCG C-3Ј; and R1367A, 5Ј-CTG CAC GAC CTC GCC TTC AAG ATC AC-3Ј.
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ABCC1 p.Arg1367Ala 21177244:51:282
status: NEW158 To test this hypothesis, mutants H1049A, H1364A, and R1367A were generated, and the levels of the mutant proteins in HEK293T cells determined as described above.
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ABCC1 p.Arg1367Ala 21177244:158:53
status: NEW159 As observed for the CL5 mutant K516A, the NBD2 mutant R1367A was not detectable by immunoblot analysis (Fig. 5C).
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ABCC1 p.Arg1367Ala 21177244:159:54
status: NEW178 Homology models and levels of MRP1 mutants H1049A, H1364A, and R1367A.
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ABCC1 p.Arg1367Ala 21177244:178:63
status: NEW