ABCC1 p.His1049Ala
[switch to full view]Comments [show]
None has been submitted yet.
PMID: 21177244
[PubMed]
Iram SH et al: "Expression and function of human MRP1 (ABCC1) is dependent on amino acids in cytoplasmic loop 5 and its interface with nucleotide binding domain 2."
No.
Sentence
Comment
49
To create the CL6 mutation H1049A, a 2-kb XmaI fragment (containing nucleotides 2337-4322) in pGEM-3Z (Promega, Madison, WI) was used as the template with the mutagenic primer 5Ј-CCG CTG TCT GGC CGT GGA CCT GC-3Ј.
X
ABCC1 p.His1049Ala 21177244:49:27
status: NEW158 To test this hypothesis, mutants H1049A, H1364A, and R1367A were generated, and the levels of the mutant proteins in HEK293T cells determined as described above.
X
ABCC1 p.His1049Ala 21177244:158:33
status: NEW160 The levels of the NBD2 mutant H1364A and the CL6 mutant H1049A were also reduced by Ͼ50%.
X
ABCC1 p.His1049Ala 21177244:160:56
status: NEW178 Homology models and levels of MRP1 mutants H1049A, H1364A, and R1367A.
X
ABCC1 p.His1049Ala 21177244:178:43
status: NEW233 If such interdomain interactions exist and are important for the proper folding and assembly of MRP1, it was reasonable to expect that, as we observed for the Ala substitution of Glu535 , replacing His1049 with Ala would also affect the levels of MRP1.
X
ABCC1 p.His1049Ala 21177244:233:198
status: NEW234 Indeed, this was the case, although the reduction in E535A protein levels was significantly greater (Ͼ90%) than the reduction observed for H1049A (60%).
X
ABCC1 p.His1049Ala 21177244:234:145
status: NEW