ABCC1 p.Val680Thr
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PMID: 19063607
[PubMed]
Qin L et al: "Residues responsible for the asymmetric function of the nucleotide binding domains of multidrug resistance protein 1."
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Sentence
Comment
55
Additional mutations (W653Y, V680T, P794A, V1432G, L1437R, and C1439S) were introduced into the dh D793E vector by site-directed mutagenesis using a QuikChange II kit (Stratagene, La Jolla, CA).
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ABCC1 p.Val680Thr 19063607:55:29
status: NEW56 The forward primers for W653Y, V680T, P794A, V1432G, L1437R, and C1439S were 5'-GCCACATTCACCTATGCCAGGAGC- GAC-3', 5'-GTGGTGGGCCAGACGGGCTGCGGAAAG-3', 5'-TTTA- CCTTCGATGAGGCCCTCTCAGCAGTGGA-3', 5'-AGAAC- CTCAGTGGCGGGCAGCGC-3', and 5'-CAGCGCCAGC- GTGTGAGCCTAGCCCG-3', respectively.
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ABCC1 p.Val680Thr 19063607:56:31
status: NEW58 Briefly, to make W653Y, V680T, and P794A mutations, a BamHI-XbaI fragment from the dh D793E construct was cloned into pBluescript II KS (+) vector (Fermentas International Inc., Burlington, Ontario, Canada), and mutagenesis PCR reactions were performed according to the manufacturer`s manual.
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ABCC1 p.Val680Thr 19063607:58:24
status: NEW148 The Walker A V680T mutation and the signature C V1432G mutation in NBD2 were without effect, while the double L1437R/C1439S signature C mutation essentially inactivated the D793E protein (Figure 3B).
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ABCC1 p.Val680Thr 19063607:148:13
status: NEW180 (A) Immunoblot of membrane vesicle proteins isolated from Sf21 cells expressing wt or mutant dual halves of MRP1 (dh D793E, dh W653Y/D793E, dh V680T/D793E, dh D793E/P794A, dh D793E/V1432G, and dh D793E/L1437R/C1439S) or Sf21 cells infected with a control vector expressing -gus.
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ABCC1 p.Val680Thr 19063607:180:143
status: NEW