ABCMdb

ABCC1 p.Pro1150Val

None has been submitted yet.

Publications

PMID: 18336795 [PubMed] Letourneau IJ et al: "Role of proline 1150 in functional interactions between the membrane spanning domains and nucleotide binding domains of the MRP1 (ABCC1) transporter."

No. Sentence Comment

52 Mutagenesis was performed according to the manufacturer`s instructions with the following sense primers (the substituted nucleotides causing the mutation are underlined; silent nucleotide substitutions added to introduce or disrupt a restriction site are in bold; other nucleotide substitutions are in lowercase typeface; and diagnostic restriction enzymes are indicated in parentheses): MRP1-Pro1150Gly (50 -G TCG GTC AGC CGG TCg GGG GTC TAT TCC C-30 ) (BsrFI), MRP1-Pro1150Ile (50 -C AGC CGC TCC ATC GTC TAC TCC CAT TTC AAC-30 ) (AccI), MRP1- Pro1150Leu (50 -CG GTC AGC CGG TCC CTC GTC TAC TCC CAT TTC-30 ) (AccI) and MRP1-Pro1150Val (50 -CG GTC AGC CGG TCC GTG GTC TAT TCC C-30 ) (RsrII). Login to comment
109 Levels of [3 H]LTC4 labeling of the Pro1150Gly, Pro1150Ile, Pro1150Leu and Pro1150Val mutants were also comparable to wild-type MRP1 (Fig. 3A). Login to comment
147 Under these conditions, the Pro1150Gly, Pro1150Ile and Pro1150Leu mutants exhibited similar levels of [a32 P]8N3ADP trapping (50-70% reduction) (Fig. 5B), while trapping by the Pro1150Val mutant was only slightly reduced (20%) relative to wild-type MRP1 (Fig. 5B). Login to comment
221 Although none of the amino acids could replace Pro1150 with respect to the substrate specificity of MRP1, it is interesting that MRP1-Pro1150Val exhibited a substantially smaller decrease in vanadate-induced [a32 P]8N3ADP trapping than the other mutants. Login to comment

PMID: 21143116 [PubMed] He SM et al: "Structural and functional properties of human multidrug resistance protein 1 (MRP1/ABCC1)."

No. Sentence Comment

758 The Pro1150Gly, Pro1150Ile, Pro1150Leu and Pro1150Val mutants exhibited a phenotype similar to the Pro1150Ala mutant with respect to organic anion transport and [ 32 P]8N3ATP photolabeling [347]. Login to comment