ABCMdb

ABCC1 p.His1486Gln

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Publications

PMID: 17187755 [PubMed] Yang R et al: "Hydrogen-bond formation of the residue in H-loop of the nucleotide binding domain 2 with the ATP in this site and/or other residues of multidrug resistance protein MRP1 plays a crucial role during ATP-dependent solute transport."

No. Sentence Comment

43 In addition, the following mutations as shown in Fig. 5A (H827D, H1486D, H827D/ H1486D, H827N, H1486N, H827N/H1486N, H827E, H1486E, H827E/ H1486E, H827Q, H1486Q, H827Q/H1486Q, H827Y, H1486Y, H827Y/ H1486Y, H827W, H1486W and H827W/H1486W) were also introduced into the full length MRP1 cDNA by using the following primers: H827D/forward, 5'-CGG ATC TTG GTC ACG GAC AGC ATG AGC TAC TTG-3'; H827D/ reverse, 5'-CAA GTA GCT CAT GCT GTC CGT GAC CAA GAT CCG-3'; H1486D/forward, 5'-GTC CTC ACC ATC GCC GAC CGG CTC AAC ACC ATC-3'; H1486D/reverse, 5'-GAT GGT GTT GAG CCG GTC GGC GAT GGT GAG GAC-3'; H827N/forward, 5'-CGG ATC TTG GTC ACG AAC AGC ATG AGC TAC TTG-3'; H827N/reverse, 5'-CAA GTA GCT CAT GCT GTT CGT GAC CAA GAT CCG-3'; H1486N/forward, 5'-GTC CTC ACC ATC GCC AAC CGG CTC AAC ACC ATC-3'; H1486N/reverse, 5'-GAT GGT GTT GAG CCG GTT GGC GAT GGT GAG GAC-3'; H827E/forward, 5'-CGG ATC TTG GTC ACG GAG AGC ATG AGC TAC TTG-3'; H827E/reverse, 5'-CAA GTA GCT CAT GCT CTC CGT GAC CAA GAT CCG-3'; H1486E/forward, 5'-GTC CTC ACC ATC GCC GAG CGG CTC AAC ACC ATC-3'; H1486E/reverse, 5'-GAT GGT GTT GAG CCG CTC GGC GAT GGT GAG GAC-3'; H827Q/forward, 5'- CGG ATC TTG GTC ACG CAG AGC ATG AGC TAC TTG-3'; H827Q/ reverse, 5'-CAA GTA GCT CAT GCT CTG CGT GAC CAA GAT CCG-3'; H1486Q/forward, 5'-GTC CTC ACC ATC GCC CAG CGG CTC AAC ACC ATC-3'; H1486Q/reverse, 5'-GAT GGT GTT GAG CCG CTG GGC GAT GGT GAG GAC-3'; H827Y/forward, 5'-CGG ATC TTG GTC ACG TAC AGC ATG AGC TAC TTG-3'; H827Y/reverse, 5'-CAA GTA GCT CAT GCT GTA CGT GAC CAA GAT CCG-3'; H1486Y/forward, 5'-GTC CTC ACC ATC GCC TAC CGG CTC AAC ACC ATC-3'; H1486Y/reverse, 5'-GAT GGT GTT GAG CCG GTA GGC GAT GGT GAG GAC-3'; H827W/forward, 5'-CGG ATC TTG GTC ACG TGG AGC ATG AGC TAC TTG-3'; H827W/reverse, 5'-CAA GTA GCT CAT GCT CCA CGT GAC CAA GAT CCG-3'; H1486W/forward, 5'-GTC CTC ACC ATC GCC TGG CGG CTC AAC ACC ATC-3'; H1486W/reverse, 5'-GAT GGT GTT GAG CCG CCA GGC GAT GGT GAG GAC-3'. Login to comment
164 In order to test this hypothesis, H827D, H1486D, H827D/H1486D, H827N, H1486N, H827N/H1486N, H827E, H1486E, H827E/H1486E, H827Q, H1486Q, H827Q/H1486Q, H827Y, H1486Y, H827Y/ H1486Y, H827W, H1486W and H827W/H1486W mutations (Fig. 5A) were introduced into full length of MRP1 cDNA in pNUT/MRP1/His and expressed in BHK cells at 37 °C. Login to comment
168 For example, H1486Q had similar transport activity (112%) as that of wild-type MRP1 (Fig. 5C), whereas H1486E dropped this activity to ~36% of the wild-type (Fig. 5C). Login to comment
210 In contrast, mutations in NBD2, such as H1486L, H1486F, H1486D, H1486N, H1486E, H1486Q, H1486Y and H1486W, have variant effects on the ATP-dependent LTC4 transport (Figs. 1C, 4B and 5C)). Login to comment
223 In contrast, substitution of the histidine residue in H-loop with an amino acid that might potentially form the hydrogen-bonds should support the ATP hydrolysis and ATP-dependent solute transport. Indeed, H1486Q-mutated MRP1 is fully active (Fig. 5C), implying that the hydrogen-bond formations between Q1486 and the γ-phosphate of the bound ATP or the putative catalytic base E1455 are as strong as in wild-type. Login to comment
224 However, H1486E exerts ~36% of wild-type MRP1 transport activity (Fig. 5C), presumably the carboxyl group of E1486 cannot form strong hydrogen-bonds with the γ-phosphate of the bound ATP and the putative catalytic base E1455 in NBD2. Accordingly, although H1486N has the same side group as the H1486Q mutation, except that the length of the side chain in H1486N is one carbon (or 1.541 Å) shorter than in H1486Q, the transport activity of H1486N dropped from ~112% (H1486Q) to ~60% (Fig. 5C), implying that the distance between two groups forming hydrogen-bond also plays an important role. Login to comment