ABCC1 p.Tyr568Ser
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PMID: 16415113
[PubMed]
Zhang DW et al: "Mutational analysis of polar amino acid residues within predicted transmembrane helices 10 and 16 of multidrug resistance protein 1 (ABCC1): effect on substrate specificity."
No.
Sentence
Comment
34
In TM10, Thr550 , Thr552 , Thr556 , Thr564 , and Thr570 were individually mutated to Ala, and Tyr568 was mutated to Ala, Ser, Phe, and Trp.
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ABCC1 p.Tyr568Ser 16415113:34:94
status: NEW48 Mutations T550A, T552A, T556A, Y568A, Y568S, Y568F, Y568W, T570A, and N1208A were generated using the Quikchange Site-Directed Mutagenesis kit (Stratagene, La Jolla, CA).
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ABCC1 p.Tyr568Ser 16415113:48:38
status: NEW50 Oligonucleotides bearing mismatched bases at the residues to be mutated (underlined) were synthesized by ACGT Corp. with the following sequences: T550A, 5Ј-GTCAGCCGTGGG- CGCCTTCACCTGGGT-3Ј; T552A, 5Ј-CGTGGGCACCTTCGCCTGGGTC- TGCAC-3Ј; T556A, 5Ј-CACCTGGGTCTGCGCGCCCTTTCTGGT-3Ј; Y568A, 5Ј-TGCACATTTGCCGTCGCCGTGACCATTGACGA-3Ј; Y568S, 5Ј-TGCACATTTGCCGTCTCCGTGACCATTGACGA-3Ј; Y568F, 5Ј-TGC- ACATTTGCCGTCTTCGTGACCATTGACGA-3Ј; Y568W, 5Ј-TGCACAT- TTGCCGTCTGGGTGACCATTGACGA-3Ј; T570A, (5Ј-TGCCGTCTACG- TGGCCATTGACGAGAACAAC-3Ј; and N1208A, 5Ј-GCCGTGCGGCTG- GAGTGTGTGGGCGCC-3Ј.
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ABCC1 p.Tyr568Ser 16415113:50:371
status: NEW121 Effect of Mutations Y568S, Y568F, and Y568W on the Transport of [3 H]LTC4 and [3 H]E217betaG by MRP1.
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ABCC1 p.Tyr568Ser 16415113:121:20
status: NEW126 Like mutation Y568A, substitution of Tyr568 with Ser did not affect the transport of LTC4 but decreased E217betaG transport.
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ABCC1 p.Tyr568Ser 16415113:126:37
status: NEW