ABCC1 p.Leu1515Ala

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PMID: 15459206 [PubMed] Westlake CJ et al: "Identification and characterization of functionally important elements in the multidrug resistance protein 1 COOH-terminal region."
No. Sentence Comment
227 The L1497A/I1498A/V1499A/L1500A mutation essentially inactivated the protein, and the double L1514A/L1515A mutation reduced transport by 80%.
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ABCC1 p.Leu1515Ala 15459206:227:100
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228 Similar to the MRP11-1527, the L1514A/L1515A mutant had a 4-fold increase in the Km for ATP and an approximate 2.5-fold decrease in the Vmax for LTC4 transport.
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ABCC1 p.Leu1515Ala 15459206:228:38
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256 Related structure COOH-terminal mutation Expression (relative to WT) LTC4 uptake (percentage of WT) Km Vmax -fold % ␮M pmol mg-1 min-1 Helix C3 L1488A 1.2 48 Ϯ 2 I1491A 1.2 59 Ϯ 2 M1492A 1.4 89 Ϯ 3 Sheet C2 V1499A 1.0 94 Ϯ 6 L1500A 1.1 22 Ϯ 1 V1497A/I1498A/V1499A/L1500A 1.0 3.6 Ϯ 0.8 D1501A 1.0 90 Ϯ 5 Sheet C1 G1503A 0.8 100 Ϯ 4 I1505A 0.5 90 Ϯ 10 E1507A 0.8 108 Ϯ 6 G1509A 0.8 113 Ϯ 9 Helix C2 P1511A 1.0 82 Ϯ 6 L1514A/L1515A 1.2 18 Ϯ 2 623 84 L1514V/L1515V 1.0 75 Ϯ 1 L1514I/L1515I 1.0 94 Ϯ 2 Q1516A 1.2 94 Ϯ 3 R1518A 0.8 103 Ϯ 10 G1519A 1.1 100 Ϯ 10 L1520A 1.3 110 Ϯ 3 Helix C1 F1521A 0.8 38 Ϯ 1 F1521Y 1.0 105 Ϯ 2 F1521G 0.9 40 Ϯ 1 F1521Q 1.0 30 Ϯ 1 F1521E 1.0 22 Ϯ 1 Y1522A 1.2 90 Ϯ 5 S1523A 1.1 103 Ϯ 6 M1524A 1.0 130 Ϯ 2 M1524G 1.0 65 Ϯ 3 A1525G 1.0 100 Ϯ 4 M1524G/A1525G 1.0 21 Ϯ 3 733 68 K1526A 2.5 85 Ϯ 2 D1527A/G1529A 3.0 123 Ϯ 4 tation impaired nucleotide binding and trapping at NBD2 to an extent comparable with that resulting from removal of the COOH-terminal 4 amino acids (not shown).
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ABCC1 p.Leu1515Ala 15459206:256:501
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PMID: 15772158 [PubMed] Westlake CJ et al: "Role of the NH2-terminal membrane spanning domain of multidrug resistance protein 1/ABCC1 in protein processing and trafficking."
No. Sentence Comment
162 MRP11-203-CFP restored plasma membrane localization both to MRP1204 -1501, MRP1204 -1527 and the double Leu to Ala mutation, MRP1204 -1531L1514A-L1515A, with resultant colocalization of the two fragments (Figure 7).
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ABCC1 p.Leu1515Ala 15772158:162:145
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