ABCC1 p.Asn590Asp
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PMID: 15260484
[PubMed]
Zhang DW et al: "Transmembrane helix 11 of multidrug resistance protein 1 (MRP1/ABCC1): identification of polar amino acids important for substrate specificity and binding of ATP at nucleotide binding domain 1."
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Sentence
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60
Mutations S585A, N590A, N590Q, N590D, N597A, and S604A were generated using the QuikchangeSite-Directed Mutagenesis kit (STRATAGENE, La Jolla, CA).
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ABCC1 p.Asn590Asp 15260484:60:31
status: NEW68 CGG TTT CCC-3'), N590D (5'-CT TTG GCC TTG TTC GAT ATC CTC CGG TTT CCC-3'), N597A (5'-CTC CGG TTT CCC CTG GCC ATT CTC CCC ATG GT-3'), and S604A (5'-CTC CCC ATG GTC ATC GCC AGC ATC GTG CAG GC-3') After confirming all mutations by DNA Thermo Sequenase Cy5.5 and Cy5.0 dye terminator cycle sequencing (Amersham Biosciences) according to the manufacturer`s instructions, DNA fragments containing the desired mutations were transferred into pCEBV7-MRP1.
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ABCC1 p.Asn590Asp 15260484:68:17
status: NEW158 Effect of Mutations S604T, N590Q, and N590D on Transport of [3 H]LTC4 and [3 H]E217 G by Wild-Type MRP1.
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ABCC1 p.Asn590Asp 15260484:158:38
status: NEW161 Ser604 was mutated to Thr, and Asn590 was converted to Asp and Gln.
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ABCC1 p.Asn590Asp 15260484:161:31
status: NEW165 Mutations N590Q, N590D, and S604T had no effect on transport of the two substrates.
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ABCC1 p.Asn590Asp 15260484:165:17
status: NEW205 On the other hand, despite the fact that converting Asn590 to either Gln or Asp had no effect on the drug resistance profile of MRP1, substitution of the residue with Ala significantly decreased resistance to vincristine, doxorubicin, and VP-16.
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ABCC1 p.Asn590Asp 15260484:205:52
status: NEW