ABCC1 p.Ser604Thr
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PMID: 15260484
[PubMed]
Zhang DW et al: "Transmembrane helix 11 of multidrug resistance protein 1 (MRP1/ABCC1): identification of polar amino acids important for substrate specificity and binding of ATP at nucleotide binding domain 1."
No.
Sentence
Comment
55
Mutations Q580A, T581A, S604T, and S605A were generated using the Transformer- Site-Directed Mutagenesis kit (CLONTECH, Palo Alto, CA).
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ABCC1 p.Ser604Thr 15260484:55:24
status: NEW59 They are as follows: Q580A (5'-C ATC CTG GAT GCC GCG ACG GCC TTC GTG TC-3'), T581A (5'-CTG GAT GCC CAG GCG GCC TTC GTG TCT TTG-3'), S605A (5'-C ATG GTC ATC AGC GCG ATC GTG CAG GCG-3'), and S604T (5'-CCC ATG GTC ATC ACT AGT ATC GTG CAG GCG-3').
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ABCC1 p.Ser604Thr 15260484:59:189
status: NEW158 Effect of Mutations S604T, N590Q, and N590D on Transport of [3 H]LTC4 and [3 H]E217 G by Wild-Type MRP1.
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ABCC1 p.Ser604Thr 15260484:158:20
status: NEW161 Ser604 was mutated to Thr, and Asn590 was converted to Asp and Gln.
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ABCC1 p.Ser604Thr 15260484:161:0
status: NEW165 Mutations N590Q, N590D, and S604T had no effect on transport of the two substrates.
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ABCC1 p.Ser604Thr 15260484:165:28
status: NEW203 Mutations Q580A, T581A, S585A, S604A, and S604T had no significant effect on the ability of MRP1 to confer resistance to any drug tested.
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ABCC1 p.Ser604Thr 15260484:203:42
status: NEW270 In addition, although mutation S604T had no effect on any of the MRP1 functions tested, converting Ser604 to Ala decreased the apparent Km values and increased Vmax for E217 G uptake.
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ABCC1 p.Ser604Thr 15260484:270:31
status: NEW