ABCC1 p.Asn590Gln
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PMID: 15260484
[PubMed]
Zhang DW et al: "Transmembrane helix 11 of multidrug resistance protein 1 (MRP1/ABCC1): identification of polar amino acids important for substrate specificity and binding of ATP at nucleotide binding domain 1."
No.
Sentence
Comment
47
Ser604 was converted to Ala and Thr, and Asn590 was mutated to Ala, Gln, and Asp.
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ABCC1 p.Asn590Gln 15260484:47:41
status: NEW60 Mutations S585A, N590A, N590Q, N590D, N597A, and S604A were generated using the QuikchangeSite-Directed Mutagenesis kit (STRATAGENE, La Jolla, CA).
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ABCC1 p.Asn590Gln 15260484:60:24
status: NEW64 They are as follows: S585A (5'-C CAG ACA GCC TTC GTG GCT TTG GCC TTG-3), N590A (5'- CT TTG GCC TTG TTC GCC ATC CTC CGG TTT CCC-3'), N590Q (5'-CT TTG GCC TTG TTC CAG ATC CTC FIGURE 1: Topology of human MRP1.
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ABCC1 p.Asn590Gln 15260484:64:132
status: NEW158 Effect of Mutations S604T, N590Q, and N590D on Transport of [3 H]LTC4 and [3 H]E217 G by Wild-Type MRP1.
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ABCC1 p.Asn590Gln 15260484:158:27
status: NEW165 Mutations N590Q, N590D, and S604T had no effect on transport of the two substrates.
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ABCC1 p.Asn590Gln 15260484:165:10
status: NEW205 On the other hand, despite the fact that converting Asn590 to either Gln or Asp had no effect on the drug resistance profile of MRP1, substitution of the residue with Ala significantly decreased resistance to vincristine, doxorubicin, and VP-16.
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ABCC1 p.Asn590Gln 15260484:205:52
status: NEW