ABCC1 p.Ser585Ala
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PMID: 15260484
[PubMed]
Zhang DW et al: "Transmembrane helix 11 of multidrug resistance protein 1 (MRP1/ABCC1): identification of polar amino acids important for substrate specificity and binding of ATP at nucleotide binding domain 1."
No.
Sentence
Comment
5
Mutations Q580A, T581A, and S585A in the predicted outer leaflet region of the helix had no detectable effect on function, while mutation of three residues close to the membrane/cytoplasm interface altered substrate specificity.
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ABCC1 p.Ser585Ala 15260484:5:28
status: NEW60 Mutations S585A, N590A, N590Q, N590D, N597A, and S604A were generated using the QuikchangeSite-Directed Mutagenesis kit (STRATAGENE, La Jolla, CA).
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ABCC1 p.Ser585Ala 15260484:60:10
status: NEW64 They are as follows: S585A (5'-C CAG ACA GCC TTC GTG GCT TTG GCC TTG-3), N590A (5'- CT TTG GCC TTG TTC GCC ATC CTC CGG TTT CCC-3'), N590Q (5'-CT TTG GCC TTG TTC CAG ATC CTC FIGURE 1: Topology of human MRP1.
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ABCC1 p.Ser585Ala 15260484:64:21
status: NEW151 The levels of LTC4 uptake by vesicles prepared from HEK transfectants expressing either wild-type MRP1 or mutations Q580A, T581A, S585A, N597A, S604A, and S605A were proportional to the relative expression levels of the wild-type and mutant proteins.
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ABCC1 p.Ser585Ala 15260484:151:130
status: NEW154 Mutations Q580A, T581A, S585A, N597A, and S605A had no detectable effect on transport.
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ABCC1 p.Ser585Ala 15260484:154:24
status: NEW203 Mutations Q580A, T581A, S585A, S604A, and S604T had no significant effect on the ability of MRP1 to confer resistance to any drug tested.
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ABCC1 p.Ser585Ala 15260484:203:24
status: NEW