ABCC1 p.Thr581Ala
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PMID: 15260484
[PubMed]
Zhang DW et al: "Transmembrane helix 11 of multidrug resistance protein 1 (MRP1/ABCC1): identification of polar amino acids important for substrate specificity and binding of ATP at nucleotide binding domain 1."
No.
Sentence
Comment
5
Mutations Q580A, T581A, and S585A in the predicted outer leaflet region of the helix had no detectable effect on function, while mutation of three residues close to the membrane/cytoplasm interface altered substrate specificity.
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ABCC1 p.Thr581Ala 15260484:5:17
status: NEW55 Mutations Q580A, T581A, S604T, and S605A were generated using the Transformer- Site-Directed Mutagenesis kit (CLONTECH, Palo Alto, CA).
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ABCC1 p.Thr581Ala 15260484:55:17
status: NEW59 They are as follows: Q580A (5'-C ATC CTG GAT GCC GCG ACG GCC TTC GTG TC-3'), T581A (5'-CTG GAT GCC CAG GCG GCC TTC GTG TCT TTG-3'), S605A (5'-C ATG GTC ATC AGC GCG ATC GTG CAG GCG-3'), and S604T (5'-CCC ATG GTC ATC ACT AGT ATC GTG CAG GCG-3').
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ABCC1 p.Thr581Ala 15260484:59:77
status: NEW151 The levels of LTC4 uptake by vesicles prepared from HEK transfectants expressing either wild-type MRP1 or mutations Q580A, T581A, S585A, N597A, S604A, and S605A were proportional to the relative expression levels of the wild-type and mutant proteins.
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ABCC1 p.Thr581Ala 15260484:151:123
status: NEW154 Mutations Q580A, T581A, S585A, N597A, and S605A had no detectable effect on transport.
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ABCC1 p.Thr581Ala 15260484:154:17
status: NEW203 Mutations Q580A, T581A, S585A, S604A, and S604T had no significant effect on the ability of MRP1 to confer resistance to any drug tested.
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ABCC1 p.Thr581Ala 15260484:203:17
status: NEW