ABCC1 p.Asp430Lys
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PMID: 15155831
[PubMed]
Haimeur A et al: "Mutations of charged amino acids in or near the transmembrane helices of the second membrane spanning domain differentially affect the substrate specificity and transport activity of the multidrug resistance protein MRP1 (ABCC1)."
No.
Sentence
Comment
48
The sequences of the individual sense strands (with the corresponding amino acid changes indicated in parentheses, the altered codons underlined, and silent mutations introducing new restriction sites italicized) were as follows: K(D)332K, 5Ј-G AGC TTC TTC TTC AAG GCC ATC CAC GAC CTG-3Ј; K332R, 5Ј-G AGC TTC TTC TTC AGG GCC ATC CAC GAC CTG-3Ј; D(K)336E, 5Ј-C AAG GCC ATC CAC GAG CTC ATG ATG TTT TCC-3Ј; D336K, 5Ј-C AAG GCC ATC CAC AAG CTT ATG ATG TTT TCC-3Ј; K332D/D336K, 5Ј-GC TTC TTC TTC GAC GCC ATC CAC AAA CTG ATG ATG-3Ј; K319D, 5Ј-G TTT AAG GTG TTA TAC GAC ACG TTT GGG CCC-3Ј; K347D, 5Ј-GGG CCG CAG ATA TTA GAC TTG CTC ATC AAG-3Ј; K347L, 5Ј-GGG CCG CAA ATC TTA CTT TTG CTC ATC AAG-3Ј; D360K, 5Ј-GAC ACG AAG GCG CCA AAG TGG CAG GGC TAC-3Ј; R394D, 5Ј-C GTC AGT GGC ATG GAG ATC AAG ACC GCT GTC-3Ј; R394I, 5Ј-C GTC AGT GGC ATG ATC ATC AAG ACC GCT GTC-3Ј; K396E, 5Ј-GT GGC ATG AGG ATC GAG ACC GCT GTC ATT GGG-3Ј; K396I, 5Ј-GT GGC ATG AGG ATC ATC ACC GCT GTC ATT GGG-3Ј; K(E)396R, 5Ј-GGC ATG AGG ATC AGG ACC GCT GTC ATT GGG GC-3Ј; D430K, 5Ј-C AAC CTC ATG TCT GTG AAG GCT CAG AGG Fig. 1.
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ABCC1 p.Asp430Lys 15155831:48:1199
status: NEW96 The exception was the Asp430 mutant D430K which, like endogenous MRP1 in vector control membrane vesicles, was undetectable under the conditions used.
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ABCC1 p.Asp430Lys 15155831:96:36
status: NEW161 When Asp430 was replaced with Lys, the resulting D430K mutant was not expressed (Fig. 2).
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ABCC1 p.Asp430Lys 15155831:161:5
status: NEWX
ABCC1 p.Asp430Lys 15155831:161:49
status: NEW232 The exception was the Asp430 mutant D430K.
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ABCC1 p.Asp430Lys 15155831:232:36
status: NEW233 Northern blot analyses showed that the D430K-expressing cells contained levels of the mutant MRP1 mRNA that were comparable with those of wild-type MRP1 transfected cells, indicating a post-transcriptional event is probably involved (results not shown).
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ABCC1 p.Asp430Lys 15155831:233:39
status: NEW