ABCC1 p.His335Glu
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PMID: 12186871
[PubMed]
Haimeur A et al: "Charged amino acids in the sixth transmembrane helix of multidrug resistance protein 1 (MRP1/ABCC1) are critical determinants of transport activity."
No.
Sentence
Comment
47
The sequences of the individual sense strands, with the altered codons underlined and the corresponding changes in amino acids indicated in parentheses were as follows: (K332D) 5Ј-CTC ATG AGC TTC TTC TTC GAC GCC ATC CAC GAC CTG-3Ј; (K332L) 5Ј-CTC ATG AGC TTC TTC TTC CTG GCC ATC CAC GAC CTG-3Ј; (H335E) 5Ј-GC TTC TTC TTC AAG GCC ATC GAG GAC CTG ATG ATG-3Ј; (H335L) 5Ј-GC TTC TTC TTC AAG GCC ATC TTG GAC CTG ATG ATG-3Ј; (H335Q) 5Ј-GC TTC TTC TTC AAG GCC ATC CAG GAC CTG ATG ATG-3Ј; (D336R) 5Ј-C AAG GCC ATC CAC CGG CTG ATG ATG TTT TCG-3Ј; (D336L) 5Ј-C AAG GCC ATC CAC CTG CTG ATG ATG TTT TCG-3Ј.
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ABCC1 p.His335Glu 12186871:47:320
status: NEW104 Representative confocal micrographs of cells expressing GFP-tagged wild-type MRP1 and mutants K332D, H335E, and D336R are shown in Fig. 2.
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ABCC1 p.His335Glu 12186871:104:101
status: NEW108 Thus H335E and H335L and H335Q all transported LTC4 at levels that were ϳ50-60% of wild-type MRP1 uptake levels (Fig. 3C).
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ABCC1 p.His335Glu 12186871:108:5
status: NEW110 Kinetic Analysis of [3 H]LTC4 Uptake in His335 Mutant MRP1-enriched Membrane Vesicles-To further investigate the effect of the His335 substitutions on the reduced ability of MRP1 to transport LTC4, the kinetic parameters of [3 H]LTC4 uptake by the H335E, H335L, and H335Q MRP1 mutants were determined (Fig. 4).
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ABCC1 p.His335Glu 12186871:110:248
status: NEW113 Thus, for wild-type MRP1, the Vmax (LTC4) was 101 pmol-1 mg-1 min-1 compared with 69, 63, and 48 pmol-1 mg-1 min-1 for the H335E, H335L, FIG. 1.
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ABCC1 p.His335Glu 12186871:113:123
status: NEW131 In contrast, LTC4 had very little effect (Ͻ15%) on E217betaG uptake by MRP1 mutants K332D and K332L, indicating that loss of LTC4 transport in these mutants is associated with a loss of binding of this substrate. On the other hand, LTC4 was still able to inhibit E217betaG uptake by MRP1 mutants H335E, H335L, and H335Q, which is consistent with only a partial reduction in LTC4 transport activity observed with these mutants (Fig. 6B).
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ABCC1 p.His335Glu 12186871:131:302
status: NEW141 C, wild-type MRP1 (f); MRP1 mutants H335E (ƒ), H335L (Ⅺ), and H335Q (q); and control pcDNA3.1(-) vector (E).
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ABCC1 p.His335Glu 12186871:141:36
status: NEW145 Kinetics of [3 H]LTC4 uptake by wild-type MRP1 and MRP1 TM6 mutants H335E, H335L, and H335Q.
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ABCC1 p.His335Glu 12186871:145:68
status: NEW146 A, shown is the Michaelis-Menten plot of the initial rate of ATP-dependent [3 H]LTC4 uptake by membrane vesicles prepared from HEK293T cells transfected with wild-type MRP1 (f) or MRP1 His335 mutants H335E (Ⅺ), H335L (ƒ), and H335Q (q).
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ABCC1 p.His335Glu 12186871:146:200
status: NEW164 B, time courses of [3 H]E217betaG uptake by wild-type MRP1 (f); TM6 mutants H335E (ƒ), H335L (Ⅺ), and H335Q (q); and the empty pcDNA3.1(-) vector control (E).
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ABCC1 p.His335Glu 12186871:164:76
status: NEW171 B, [3 H]E217betaG uptake by wild-type MRP1 (WT-MRP1) (open bars); TM6 mutants H335E, H335L, and H335Q (shaded bars); and the empty pcDNA3.1(-) vector control (solid bars).
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ABCC1 p.His335Glu 12186871:171:78
status: NEW184 Similarly, MTX uptake by the H335E, H335L, and H335Q MRP1 mutants was comparable with wild-type MRP1.
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ABCC1 p.His335Glu 12186871:184:29
status: NEW204 Membrane vesicles were preincubated with acivicin and then incubated with [3 H]GSH in the presence of 30 M apigenin in transport buffer for 20 min at 37 °C. A, K332D and K332L; B, H335E, H335L, and H335Q; C, D336L and D336R.
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ABCC1 p.His335Glu 12186871:204:193
status: NEW207 E, WT-MRP1 (f); H335E (ƒ); H335L (Ⅺ); H335Q (q); vector control (E).
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ABCC1 p.His335Glu 12186871:207:16
status: NEW