ABCMdb

ABCG8 p.Arg111Met

None has been submitted yet.

Publications

PMID: 16352607 [PubMed] Zhang DW et al: "Functional asymmetry of nucleotide-binding domains in ABCG5 and ABCG8."

No. Sentence Comment

53 For G8, R111M-forward (5Ј-TCA GGC TGC GGG ATG GCC TCA CTA CTC-3Ј), R111M- reverse (5ЈGAG TAG TGA GGC CAT CCC GCA GCC TGA-3Ј), R111K-forward (5Ј-TCA GGC TGC GGG AAG GCC TCA CTA CTC-3Ј), R111K-reverse (5ЈGAG TAG TGA GGC CTT CCC GCA GCC TGA-3Ј), G216D-forward (5Ј-GGG GTG TCC GGG GAT GAG CGC CGA CGA-3Ј), G216D-reverse (5Ј-TCG TCG GCG CTC ATC CCC GGA CAC CCC-3Ј), V214IG215S-forward (5Ј-TAT GTA CGT GGG ATC TCC TCG GGT GAG CGC CGA-3Ј), V214IG215S-reverse (5Ј- TCG GCG CTC ACC CGA GGA GAT CCC ACG TAC ATA-3Ј), E238D-forward (5Ј-CTC ATT CTG GAT GAC CCC ACT TCT GGC-3Ј), E238D-reverse (5Ј-GCC AGA AGT GGG GTC ATC CAG AAT GAG-3Ј), E238Q-forward (5Ј-CTC ATT CTG GAT CAG CCC ACT TCT GGC-3Ј), and E238D-reverse (5Ј-GCC AGA AGT GGG CTG ATC CAG AAT GAG-3Ј). Login to comment
156 In contrast to these results, replacement of the corresponding basic residue in the Walker A motif of G8 with methionine (R111M) or with lysine (R111K) had no effect on either the amount of immunodetectable G5 or G8 or on the ability of G5 or G8 to bind ATP or trap ADP (Fig. 5A). Login to comment
174 Substitution of Arg111 in G8 to methionine or lysine had no adverse effects on biliary cholesterol or plant sterol levels, even when steady state levels of mature G5 and G8 were significantly reduced, as occurred in the mice expressing G8-R111M with wild-type G5 (Fig. 5B). Login to comment
189 Mutations in the Walker A motifs of G5 (K93M and K93R) and G8 (R111M and R111K); effects on protein mass, nucleotide binding and trapping in cultured cells (A) and biliary cholesterol and plant sterol secretion in vivo (B). Login to comment