ABCG2 p.Ser384Arg
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41
Mutagenesis and transfection The R383A, R383G, R383H, R383K, and R383G/S384R mutants were generated by site-directed mutagenesis in the pcDNA3.1/Myc-HisA(-) vector (Invitrogen) as previously described [26].
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ABCG2 p.Ser384Arg 19406100:41:71
status: VERIFIED183 We postulated that this arginine might function as an anchor of the first transmembrane alpha helix, thus more conservative substitutions were performed: mutation to the positively charged lysine and introduction of arginine at position 384 instead of 383, creating the R383K and R383G/S384R mutants, followed by stable transfections in HEK 293 cells.
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ABCG2 p.Ser384Arg 19406100:183:286
status: VERIFIED195 On the other hand, none of the R383G/S384R mutant clones was detectable either on the cell surface by flow cytometry with the 5D3 antibody or on immunoblot with the BXP-21 antibody (data not shown).
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ABCG2 p.Ser384Arg 19406100:195:37
status: VERIFIED