ABCG2 p.Arg482Pro
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PMID: 16815914
[PubMed]
Ejendal KF et al: "The nature of amino acid 482 of human ABCG2 affects substrate transport and ATP hydrolysis but not substrate binding."
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7
Six of the mutants (R482G, R482H, R482K, R482P, R482T, and R482Y) and the wild-type protein (R482wt) were selected for studies of basal and stimulated ATPase activity and photoaffinity labeling with the substrate analog [125 I]iodoarylazidoprazosin.
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ABCG2 p.Arg482Pro 16815914:7:41
status: VERIFIED62 Of these 20 possible variants, three (R482F, R482L, R482P) have not previously been described.
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ABCG2 p.Arg482Pro 16815914:62:52
status: VERIFIED71 Analysis of the substrate binding properties of wild-type and six mutant ABCG2 proteins In order to further investigate the effects of the R482X mutations, we studied the drug-binding ability of a selection of ABCG2 mutants (R482G, R482H, R482K, R482P, R482T, and R482Y) and the wild-type ABCG2 (R482wt).
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ABCG2 p.Arg482Pro 16815914:71:246
status: VERIFIED73 We selected mutants R482H, R482P, and R482Y because they are partially deficient in rhodamine 123 transport, whereas mitoxantrone transport is intact.
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ABCG2 p.Arg482Pro 16815914:73:27
status: VERIFIED75 Of these six ABCG2 mutants plus the wild-type protein, biochemical analyses of R482H and R482P have not been described previously.
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ABCG2 p.Arg482Pro 16815914:75:89
status: VERIFIED86 We analyzed expression of ABCG2 in the membranes using the monoclonal antibody BXP-21 (Fig. 5A), which shows that the R482G, R482wt, and R482T membranes used here express less ABCG2, compared with the membranes expressing the R482H, R482K, R482P, and R482Y variants.
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ABCG2 p.Arg482Pro 16815914:86:240
status: VERIFIED89 The R482G, R482P, and R482T variants are stimulated 1.9-, 2.1-, and 1.8-fold, respectively, by the addition of 20 mM prazosin.
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ABCG2 p.Arg482Pro 16815914:89:11
status: VERIFIED96 Specific [125 I]IAAP photoaffinity labeling of crude membranes derived from HeLa cells expressing wild-type ABCG2 (R482wt) and the ABCG2 variants R482G, R482H, R482K, R482P, R482T, and R482Y.
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ABCG2 p.Arg482Pro 16815914:96:167
status: VERIFIED106 Basal and drug-stimulated ATPase activity of wild-type ABCG2 (R482wt) and ABCG2 variants R482G, R482H, R482K, R482P, R482T, and R482Y.
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ABCG2 p.Arg482Pro 16815914:106:110
status: VERIFIED