ABCG2 p.Cys438Ser
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PMID: 16367905
[PubMed]
Kage K et al: "Role of Cys-603 in dimer/oligomer formation of the breast cancer resistance protein BCRP/ABCG2."
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62
Because BCRP protein expression levels in PA/C438S, PA/ C592S and PA/C608S cells were found to be remarkably decreased under non-reducing conditions (Fig. 2b), they were further examined by overexposing the immunoblot (Fig. 2c).
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ABCG2 p.Cys438Ser 16367905:62:45
status: VERIFIED67 In contrast, the PA/C43S, PA/C55S, PA/C284S and PA/C603S mutants expressed intermediate amounts of BCRP and, in PA/C438S cells, little surface expression of BCRP was observed.
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ABCG2 p.Cys438Ser 16367905:67:115
status: VERIFIED71 Hence, the observed differences in BCRP expression, particularly in the PA/C438S, PA/C592S, PA/C603S and PA/C608S cells, are not attributable to low transcript levels.
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ABCG2 p.Cys438Ser 16367905:71:75
status: VERIFIED110 PA/C592S and PA/C608S showed decreased drug resistance and the sensitivity of PA/C438S cells was similar to that of the parental PA317 cells.
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ABCG2 p.Cys438Ser 16367905:110:81
status: VERIFIED122 Under non-reducing conditions, small quantities of both monomeric and dimeric forms of BCRP could be observed for the PA/C438S, PA/C592S and PA/C608S transfectants as well as in PA/C603S cell extracts following overexposure of the blot (Fig. 2c).
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ABCG2 p.Cys438Ser 16367905:122:121
status: VERIFIED127 In the case of BCRP-C438S, which has a mutation in the second transmembrane domain, little expression of BCRP was observed by either western blotting or FACS.
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ABCG2 p.Cys438Ser 16367905:127:20
status: VERIFIED128 In addition, the SN-38 sensitivity of PA/C438S cells was equivalent to the parental PA317 cells (Fig. 5c).
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ABCG2 p.Cys438Ser 16367905:128:41
status: VERIFIED